|Millicell Organotypic Cell Culture Procedure|
|Millicell® inserts and plates|
|Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system|
Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
Biochemical and Biophysical Research Communications 2010
|Effects of cimetidine on the biological behaviors of human gastric cancer cells|
Jiang CG, Liu FR, Xu HM, Wu T, Gao J.
Zhonghua Yi Xue Za Zhi. 2006 Jul 11;86(26):1813-6 2006
|Overexpression of TIMP-1 mediated by recombinant adenovirus in hepatocellular carcinoma cells inhibits proliferation and invasion in vitro|
Xia D, Yan LN, Xie JG, Tong Y, Yan ML, Wang XP, Zhang MM, Zhao LY.
Hepatobiliary Pancreat Dis Int. 2006 Aug;5(3):409-15. 2006
|Glucose utilization by the retinal pigment epithelium: evidence for rapid uptake and storage in glycogen, followed by glycogen utilization.|
Senanayake P, Calabro A, Hu JG, Bonilha VL, Darr A, Bok D, Hollyfield JG.
Exp Eye Res. 2006 Aug;83(2):235-46. Epub 2006 May 11. 2006
|Development of mouse embryos co-cultured with polarized or non-polarized uterine epithelial cells using sequential culture media.|
Azadbakht M, Valojerdi MR, Mowla SJ.
Anim Reprod Sci. 2006 Jul 27 2006
|A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development.|
Baghaban Eslami Nejad MR, Rezazadeh Valojerdi M, Kazemi Ashtiani S.
J Exp Clin Assist Reprod. 2005 Apr 19;2(1):7. 2005
|Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways|
Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
Experimental Neurology 192 (2005); 394-406 2005
|Subcellular localisation of recombinant a and g-synuclein|
Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
Mol. Cell. Neurosci., 28 (2005); 326-334 2005
|Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord|
Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. 2005
|Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.|
Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82. 2004
|Why do the electrodes need to be replaced every six months?||In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.|
|What voltage value can I expect for my cell type?||We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.|
|What is the porosity of the Millicell Culture Plate Inserts?||The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10%|
|What is the thickness of the Millicell-CM membrane?||The thickness of the Millicell-CM memnbrane is approximately 50 um.|
|What is the difference between Millicell PC and PCF?||Both Millicells have polycarbonate membranes and are frequently used in chemotaxis and transport studies. PC's are recommended ONLY for suspension cell culture. PCF's are tissue culture treated and therefore recommended for attachment dependent cell culture.|
|What is the advantage of using Millicell for cell culture applications ?||Studies have shown that cells actually grow better on microporous membranes than on the artificially smooth surfaces of standard cell culture plasticware. We have found that cells grown on Millicell Cell Culture Plate Inserts produce higher cell densities and greater differentiation than cells grown in standard setups. Cells can be constantly bathed and fed on both their apical and basolateral surfaces and ions and molecules can pass freely across the membrane. Millicells also offer the researcher a more humane approach to animal testing since cell culturing with the units can offer alternatives to live animal studies for many toxicological studies.|
|What is the advantage of using Millicell 24 for cell culture applications?||Millicell 24 is an automation compatible platform that offers many design advantages for cell culture applications. The active surface area for growing cells is over twice as large as other 24 well plates. Features including the apical assist, filter plate feet, and basolateral access holes contribute to manual ease of use.|
|What cell types can be grown using Millicell 24?||Millicell 24 is an optimal platform for growing any adherent cell line used for permeability/transport, cell differentiation, fluorescence, toxicology, or co-culturing studies.|
|Are there optimal growth conditions for epithelial cells grown on Millicell 24?||We recommend feeding every other day (Mon, Wed, and Fri.) exchanging apical and basolateral media in a specific order. The preferred order is to aspirate basolaterally first, then apically, and add fresh medium back apically first, then basolaterally. Plates should be incubated at 37 degrees Celsius with 5-6% CO2 and 95% relative humidity.|
|Are the Millicell PCF inserts tissue culture treated on both sides of the membrane?||Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane.|
|Millicell 24 and Millicell 96|
|Millicell Culture Plate Inserts|
|Millicell-CM Low Height Culture Plate Inserts User Guide|
|Millicell-ERS User Guide|