|Millicell Organotypic Cell Culture Procedure|
|Millicell® inserts and plates|
|Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system|
Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
Biochemical and Biophysical Research Communications 2010
|Effects of cimetidine on the biological behaviors of human gastric cancer cells|
Jiang CG, Liu FR, Xu HM, Wu T, Gao J.
Zhonghua Yi Xue Za Zhi. 2006 Jul 11;86(26):1813-6 2006
|Overexpression of TIMP-1 mediated by recombinant adenovirus in hepatocellular carcinoma cells inhibits proliferation and invasion in vitro|
Xia D, Yan LN, Xie JG, Tong Y, Yan ML, Wang XP, Zhang MM, Zhao LY.
Hepatobiliary Pancreat Dis Int. 2006 Aug;5(3):409-15. 2006
|Glucose utilization by the retinal pigment epithelium: evidence for rapid uptake and storage in glycogen, followed by glycogen utilization.|
Senanayake P, Calabro A, Hu JG, Bonilha VL, Darr A, Bok D, Hollyfield JG.
Exp Eye Res. 2006 Aug;83(2):235-46. Epub 2006 May 11. 2006
|Development of mouse embryos co-cultured with polarized or non-polarized uterine epithelial cells using sequential culture media.|
Azadbakht M, Valojerdi MR, Mowla SJ.
Anim Reprod Sci. 2006 Jul 27 2006
|Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways|
Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
Experimental Neurology 192 (2005); 394-406 2005
|Subcellular localisation of recombinant a and g-synuclein|
Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
Mol. Cell. Neurosci., 28 (2005); 326-334 2005
|Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord|
Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. 2005
|A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development.|
Baghaban Eslami Nejad MR, Rezazadeh Valojerdi M, Kazemi Ashtiani S.
J Exp Clin Assist Reprod. 2005 Apr 19;2(1):7. 2005
|Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors|
Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein
Mol. Cell. Neurosci. , 27 (2004); 322-331 2004
|Is Millicell 24 tissue culture treated on both sides of the membrane?||Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane.|
|Are either the single well feeder tray or the 24 well receiver plates tissue culture treated?||No, they are not. If it is desired to culture adherent cells in either feeder or receiver trays, the trays must first be treated with the cell type appropriate coating (i.e. collagen).|
|What membranes are offered for Millicell 24?||Millicell 24 is offered with either a 0.4 µm polycarbonate (PC) or a 1.0 µm polyester (PET) membrane.|
|What is the advantage of using Millicell 24 for cell culture applications?||Millicell 24 is an automation compatible platform that offers many design advantages for cell culture applications. The active surface area for growing cells is over twice as large as other 24 well plates. Features including the apical assist, filter plate feet, and basolateral access holes contribute to manual ease of use.|
|What cell types can be grown using Millicell 24?||Millicell 24 is an optimal platform for growing any adherent cell line used for permeability/transport, cell differentiation, fluorescence, toxicology, or co-culturing studies.|
|Can live cells grown on Millicell 24 be viewed by microscope?||Live cells on the top of the membrane can be viewed through the media using an inverted microscope looking through the underside of the Millicell 24 with the 1.0 µm PET membrane. Live cells can also be viewed from above the filter plate with the lid on using microscope objectives with a working distance of at least 18mm.|
|Do I need an ECM for growing epithelial cells on Millicell 24?||An ECM is not necessary for many adherent cell types (i.e. epithelial cells) on Millicell 24. Tissue culture treatment of the PC and PET membranes allows for good cell attachment and growth. Specific cell types that require ECM treatment for growth or differentiation on plastic, however, may also require it for growth on filters.|
|Are there optimal growth conditions for epithelial cells grown on Millicell 24?||We recommend feeding every other day (Mon, Wed, and Fri.) exchanging apical and basolateral media in a specific order. The preferred order is to aspirate basolaterally first, then apically, and add fresh medium back apically first, then basolaterally. Plates should be incubated at 37 degrees Celsius with 5-6% CO2 and 95% relative humidity.|
|What volume of media is recommended using Millicell 24?||It is recommended to use 400 µls in the apical well and 32 mls in the single well feeder tray. When using the 24 well receiver tray we recommend using 800 µls in each tray well. The capacity volumes for both the apical well and the 24 well receiver tray (with filter well in place) is 1100 µls.|
|How do you determine optimal seeding densities of epithelial cells using Millicell 24?||This requires optimization for each laboratory due to the variability in cultures. If converting from a 0.3 cm2 surface area platform, we recommend doubling your seeding density to start and, if necessary, further optimize using a range of densities.|
|Millicell 24 and Millicell 96|
|Millicell Culture Plate Inserts|
|Millicell-CM Low Height Culture Plate Inserts User Guide|
|Millicell-ERS User Guide|