Millicell® Cell Culture Inserts

Optimized cell growth, attachment and differentiation

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Overview

Specifications

Ordering Information

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Millicell Standing Inserts Clear Sorting & Filtering
Catalogue Numbericon Mediaicon Chemistryicon Pore Sizeicon Filtration Areaicon Device Configurationicon Pack Sizeicon
PI8P01250Isopore® Polycarbonate (PC) 8.0 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PICM01250Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PICM03050Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PICM0RG50Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PIHA01250MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PIHA03050MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PIHP01250Isopore® Polycarbonate (PC) 0.4 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PIHP03050Isopore® Polycarbonate (PC) 0.4 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PITP01250Isopore® Polycarbonate (PC) 3.0 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PIXP01250Isopore® Polycarbonate (PC) 12.0 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability

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Millicell Hanging Inserts Clear Sorting & Filtering
Catalogue Numbericon Chemistryicon Pore Sizeicon Filtration Areaicon Device Configurationicon Pack Sizeicon
MCEP06H48Polyethylene Terephthalate (PET) 8.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCEP12H48Polyethylene Terephthalate (PET) 8.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCEP24H48Polyethylene Terephthalate (PET) 8.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCHT06H48Polyethylene Terephthalate (PET) 0.4 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCHT12H48Polyethylene Terephthalate (PET) 0.4 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCHT24H48Polyethylene Terephthalate (PET) 0.4 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCMP06H48Polyethylene Terephthalate (PET) 5.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCMP12H48Polyethylene Terephthalate (PET) 5.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCMP24H48Polyethylene Terephthalate (PET) 5.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCRP06H48Polyethylene Terephthalate (PET) 1.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCRP12H48Polyethylene Terephthalate (PET) 1.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCRP24H48Polyethylene Terephthalate (PET) 1.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCSP06H48Polyethylene Terephthalate (PET) 3.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCSP12H48Polyethylene Terephthalate (PET) 3.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCSP24H48Polyethylene Terephthalate (PET) 3.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability

* For attachment-dependent cells, this membrane needs to be coated with an extra cellular matrix.

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Accessories Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
MERS00002Millicell ERS-2 Voltohmmeter 1 Show Pricing & Availability
MERSSTX01Replacement Electrodes, 1 pair 1 Show Pricing & Availability

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    Documentation

    Brochure

    Title
    Advanced cell culture systems for every challenge.
    Multiwell solutions for discovery research and sample prep

    Technical Info

    Title
    Millicell Organotypic Cell Culture Procedure

    Data Sheet

    Title
    Millicell Inserts and Plates
    Millicell® inserts and plates

    References

    Reference overviewApplication
    Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system
    Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
    Biochemical and Biophysical Research Communications  2010

    Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways
    Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
    Experimental Neurology 192 (2005); 394-406  2005

    Cell Culture
    Subcellular localisation of recombinant a and g-synuclein
    Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
    Mol. Cell. Neurosci., 28 (2005); 326-334  2005

    Cell Culture
    Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord
    Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
    Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.  2005

    Cell Culture
    Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.
    Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
    Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.  2004

    Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice
    Abouelfetouh, Ayman, et al
    Brain Research (2004), Volume 1029, Issue 1 pp 114-119  2004

    Cell Culture
    Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes.
    Waldman SD, Couto DC, Omelon SJ, Kandel RA
    Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40  2004

    Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors
    Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein
    Mol. Cell. Neurosci. , 27 (2004); 322-331  2004

    Cell Culture
    FGF-10 plays an essential role in the growth of the fetal prostate.
    Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha
    Developmental Biology 261 (1): 39-54  2003

    Cell Culture
    Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge.
    J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond
    Cell Biology International 27 (1): 23-29  2003

    Cell Differentiation

    FAQ

    QuestionAnswer
    Why do the electrodes need to be replaced every six months?In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.
    What voltage value can I expect for my cell type?We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.
    What is the porosity of the Millicell Culture Plate Inserts?The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10%
    What is the thickness of the Millicell-CM membrane?The thickness of the Millicell-CM memnbrane is approximately 50 um.
    What is the difference between Millicell PC and PCF?Both Millicells have polycarbonate membranes and are frequently used in chemotaxis and transport studies. PC's are recommended ONLY for suspension cell culture. PCF's are tissue culture treated and therefore recommended for attachment dependent cell culture.
    What is the advantage of using Millicell for cell culture applications ?Studies have shown that cells actually grow better on microporous membranes than on the artificially smooth surfaces of standard cell culture plasticware. We have found that cells grown on Millicell Cell Culture Plate Inserts produce higher cell densities and greater differentiation than cells grown in standard setups. Cells can be constantly bathed and fed on both their apical and basolateral surfaces and ions and molecules can pass freely across the membrane. Millicells also offer the researcher a more humane approach to animal testing since cell culturing with the units can offer alternatives to live animal studies for many toxicological studies.
    Are the Millicell PCF inserts tissue culture treated on both sides of the membrane?Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane.
    Are the Millicell units sterile and, if so, how are they sterilized?The Millicell units are sold sterile in individual blister packs. The HA and CM units are ETO sterilized and the PC and PCF are Gamma Irradiated.
    Can cells grown on Isopore (polycarbonate) membrane be viewed under a microscope?Yes, we can observe the cells seeding on the membrane of pore size 1 um.
    Can I make membrane potential measurements with the Millicell-ERS?Yes, membrane potential measurements can be made with the ERS. Short circuit potential, however, cannot be done.

    User Guides

    Title
    Millicell 24 and Millicell 96
    Millicell Culture Plate Inserts
    Millicell Hanging Cell Culture Inserts
    Millicell-CM Low Height Culture Plate Inserts User Guide
    Millicell-ERS User Guide
    Millicell® Hanging Cell Culture Inserts