|Protein purification and preparation|
|Development of capillary electrophoresis as an alternative to high resolution agarose electrophoresis for the diagnosis of multiple sclerosis|
Sanders E.; Katzmann J.A.; Clark R.; Oda R.P.; Shihabi Z.; Landers J.P.
Clinical Chemistry and Laboratory Medicine 37/1 (37-45) 1999 1999
|The urinary light-chain ladder pattern a product of improved methodology that may complicate the recognition of Bence Jones proteinuria|
Bailey, Elizabeth M et al.
Archives of Pathology and Laboratory Medicine July, 1993; Original Article: p707| 1993
|An effective concentration method for human immunodeficiency virus type 1 (HIV-1)|
Takasaki T; Sano K; Tanaka E; Morimatsu S; Kifune K; Tsurutani R; Nakai M; Yamaguchi J
Kansenshogaku zasshi; 66 (5) p579-83, May 1992 1992
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Nozawa E. et al.,Research and Practice in Forensic Medicine 33 (0). 1990. 77-80
Research and Practice in Forensic Medicine 33 (0). 1990. 77-80 1990
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Body, Barbara A et al.
Archives of Pathology and Laboratory Medicine May, 1988; 112: 519-522 1988
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American Journal of Diseases of Children August, 1988; 142: 853-857 1988
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Copyright 1986 American Medical Association 1986
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Electrophoresis 6 (10). 1985. 504-508 1985
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Nieren- und Hochdruckkrankheiten 14/11 (484-486) 1985 1985
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|How can I dispose of a used Minicon device?||You can dispose of a Minicon by autoclaving it or incinerating it.|
|Which Minicon do I use?||The Minicon unit you chose should be based on the fluid being processed. See below.
Urine - B15 or Miniplus
Cerebrospinal Fluid - B15 or CS15
Dilute body fluid - A25
|What is the molecular weight cut-off of the Minicon unit?||The molecular weight cut-off varies with the device. The ratings for the Minicon devices are listed below.
|Will the Minicon unit concentrate to dryness?||At about 100x, the membrane has been treated to retard further concentration and accidental reduction to dryness. However, Spinal fluid and other very dilute specimens may occasionally concentrate beyond 100x. To assure recovery of valuable sample, check concentration level periodically and remove concentrate once it has reached 100x. If sample appears to go to dryness, pipette up to 0.5ml of saline or buffer into the sample chamber. Mix concentrate by drawing liquid in and out of a pipette several times before removal from chamber.|
|Are the Minicon test results quantitative?||No. There is some nonspecific binding of solute on the membrane as well as non-recoverable film loss due to wetting of the chamber that is not recoverable.|
|Can the Minicon cell be refilled? How many times?||Yes, the units can be refilled. See the following chart for specific devices. Minicon - B15 2 times Minicon - CS15 2 times Minicon - A25 3 times Miniplus 2 times|
|How many tests are there in each Minicon?||The number of tests vary with the device. See below for details.
|How much sample does each Minicon test cell hold?||The maximum cell capacities are as follows:
|What is the Minicon maximum concentration factor?||The maximum concentration factor for each device is listed below. Please note: Spinal fluid and other very dilute specimens may occasionally concentrate beyond 80X. To assure recovery of valuable samples, check concentration level periodically and remove concentrate once it has reached 80X.
Minicon B15 - 100x
Minicon CS15 - 80x
Minicon A25 - 20x
Miniplus - 200x
|Can I use Minicon after its expiration date?||It is not recommended. The flow rates will slow down after the expiration date.|