APT242 | MitoLight® Mitochondrial Apoptosis Detection Kit

APT242
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      FCFluorescent
      Description
      Catalogue NumberAPT242
      Brand Family Chemicon®
      Trade Name
      • MitoLight
      • Chemicon
      DescriptionMitoLight® Mitochondrial Apoptosis Detection Kit
      OverviewDisruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.

      MitoLight™ partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratioing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (λem = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (λem = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585).
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

      · 37°C CO2 incubator

      · Flow Cytometer

      · Glass slides with glass coverslips
      References
      Product Information
      Components
      • MitoLight™ (Part No. 71616): 100 μL in DMSO.
      • 10X Incubation Buffer (Part No. 71614): 20 mL.
      Detection methodFluorescent
      Applications
      ApplicationThe MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Mammals
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at -20°C up to their expiration date. After thawing, unused kit components can be refrozen. For best results, aliquot the MitoLight™ reagent into separate vials to avoid repeated freeze/thaw cycles.

      PROTECT REAGENTS FROM LIGHT.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Apoptosis / Cell Death Assays