EZMIL4 | Mouse IL-4 ELISA Kit, EZMIL4

EZMIL4
96-well strip plate  
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      Overview

      Replacement Information
      Replacement InformationTHIS PRODUCT HAS BEEN DISCONTINUED. PLEASE VISIT WWW.SIGMAALDRICH.COM FOR A POSSIBLE REPLACEMENT: CATALOG # RAB0299-1KT.

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-4MELISA
      Description
      Catalogue NumberEZMIL4
      DescriptionMouse IL-4 ELISA Kit, EZMIL4
      OverviewThe Mouse IL-4 ELISA Kit is a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantification of mouse IL-4 from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
      Background InformationMouse IL-4 (Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1,BSF-1, Hodgkin’s cell growth factor, HCGF, Mast cell growth factor-2, MCGF-2, Macro- phage fusion factor, MFF, T cell growth factor-2, TCGF-2) is a pleiotropic cytokine that is produced by activated T cells, mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. IL-4 regulates differentiation of naïve CD4+ T cell to Th2 cells. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. In addition, IL-4 regulates IgE and IgG1 production by B cells.
      References
      Product Information
      Precision, %
      • Intra-assay: <10 %
      • Inter-assay: <15 %
      Applications
      ApplicationThe Mouse IL-4 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody.
      Key Applications
      • ELISA
      Biological Information
      SpecificityNo cross-reactivity was observed when this kit was used to analyze mouse recombinant cytokines/chemokines at up to 50 ng/mL.
      Species Reactivity
      • Mouse
      Analytes Available
      • IL-4
      Accuracy
      • 105%
      Physicochemical Information
      Sensitivity
      • 0.5 pg/mL
      Linearity of DilutionContact Technical Service for this information
      Standard Curve Range
      • 2-125 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits