|Evaluation of MultiScreen-MIC Plates in Chemotaxis Assays|
|Evaluation of MultiScreen-MIC Plates in Invasion and Angiogenesis Assays|
|MultiScreen-MIC Plate: 96-well system for cell-based functional screening assays including migration, invasion, and chemotaxis|
|Extracellular HIV-1 Nef increases migration of monocytes|
Michael H. Lehmanna, Stefan Walterc, Loyda Ylisastiguid, Frank Striebelc, Vladimir Ovode, Matthias Geyerf, Jean Claude Gluckmand and Volker Erflea
Experimental Cell Research, Volume 312, Issue 18 , 1 November 2006, Pages 3659-3668 2006
|High throughput in vivo screening for bone anabolic compounds with zebrafish|
Angeleen Fleming, Masahiko Sato, Paul Goldsmith
Journal of Biomolecular Screening 10(8); 2005, 823-831 2005
|Screening Assay for the Identification of Deoxyhypusine Synthase Inhibitors|
Marc-Nicola Somner, Dorian Bevec, et al.
Journal of Biomolecular Screening, 2004, 9(5):434-438 2004
|Cell Based Assays|
|A cell-based assay for screening the uridine 5'-diphosphate-glucuronosyltransferase 1A inhibitory potential of new chemical entities.|
Jaime Padros, Donald Chun, Liangfu Chen, Pierre Rigolli, Themis Flarakos and Malle Jurima-Romet; Analytical Biochemistry 320 (2): 310-312
Analytical Biochemistry 320 (2): 310-312 2003
|Functional characterization of Glu298Asp mutant human endothelial nitric oxide synthase purified from a yeast expression system.|
Regina Golser, Antonius C. F. Gorren, Bernd Mayer and Kurt Schmidt
Nitric Oxide 8 (1): 7-14 2003
|Cell Based Assays|
|What is the well depth and maximum volume capacity of a MultiScreen plate?||The well depth of a 96 well MultiScreen plate is 1.245 cm. The well depth for a 384 well MultiScreen plate is 1.2 cm. The maximum working volume of a 96 well plate is 300 ul. The maximum working volume for a 384 well plate is 100 ul.|
|Assay Terminology commonly used with reference to MultiScreen-MIC plates||Chemotaxis: Directed movement of cells in response to a concentration gradient.
Migration: Random movement of cells.
Invasion: The movement of cells across a barrier (e.g. extracellular matrix) in response to a concentration gradient.
Transmigration: The movement of cells across another cell barrier in response to a concentration gradient.
Angiogenesis: Tubule formation exhibited by HUVEC (Human Umbilical Vein Endothelial Cells) in response to cell stimulating factors.
Co-culture: When you have more than one cell type in your assay it is called co-culture. These assays are typically run to assess cell-cell interactions and factors secreted by one cell influencing the behavior of the other cell.
|What type of assays can I perform on the MultiScreen-MIC plates?||he MultiScreenTM-MIC plates can be used for a variety of assays such as migration, invasion, chemotaxis, angiogenesis, transendothelial migration and co-culture type of assays.|
|What is the pore density in the MultiScreenTM-MIC plates?||Pore size Nominal pore density per cm2 3 mm MIC 2 X 106 5 mm MIC 4 X 105 8 mm MIC 1 X 105|
|Should I pre-coat the wells when using the MultiScreenTM-MIC plates?||This depends on the cell line you are using. Some cells need coating and some don’t. Again a quick literature search should help you decide the correct coating substance for your cell line.
Typical coating substances are collagen and gelatin. Extracellular matrix is typically used for coating wells when performing invasion assays and angiogenesis assays.
|How do I coat the wells when using the MultiScreenTM-MIC plates?||Use 30-50 µl volumes of coating substance at desired concentration in a buffer compatible with coating substance. Spread the solution gently across the membrane using a pipette tip taking care not to damage the membrane. An alternative approach is to pre-wet the membrane with buffer solution, aspirating and then applying the coating solution. Care must be taken to ensure that there is no drying of membrane prior to coating.|
|Can I use some wells in the MultiScreenTM-MIC plates for my assay and re-use later?||The plate is designed for single use only. We do not recommend using part of the plate, storing and setting up assay on unused wells at a later time.|
|What is the MultiScreenTM-MIC plate?||It is a 96 well sterile single use device that allows you to perform a variety of assays such as migration, invasion, chemotaxis, angiogenesis, transendothelial migration and co-culture type of assays. It has a filter plate* with 96 wells fitted with membrane. These wells serve as upper wells in your assay. It has a 96 well tear drop receiver plate. These wells serve as the lower wells in your assay. The membrane is the separation component between the two wells. These devices are available in a variety of pore sizes (3, 5 and 8 mm pore sizes available as cataloged products. 1, 2 and 12 mm pore sizes available by special order) in an uncoated format. The plates are available in packs of 10 with each device individually wrapped in a blister pack to ensure sterility. The plates are tissue culture treated (using a proprietary methodology) to support use with suspension and adherent cells.
*There is no underdrain on these filter plates, as the application does not require use of vacuum.
|What type of screening can I perform with the MultiScreenTM-MIC plates?||Some examples of screening that can be done on MIC plates are compound library screening, protein library screening or antibody library screening. You can measure the effect of the compound, protein or antibodies on cell behavior in functional assays such as migration, invasion, chemotaxis, angiogenesis, transendothelial migration and co-culture type of assays.|
|What is the surface area of membrane in the MultiScreenTM-MIC plates?||The surface area of membrane in the MultiScreenTM-MIC is 0.3 cm2|
|MultiScreen - MIC Plate|
|MultiScreen FL Plate User Guide|