12-354 Nitrotyrosine Immunoblotting Control

40 blots  
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      Replacement Information
      Catalogue Number12-354
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionNitrotyrosine Immunoblotting Control
      OverviewDescription: A combination of three proteins: nitrated bovine superoxide dismutase (SOD) ~16kDa, nitrated bovine serum albumin (BSA) ~66kDa, and nitrated rabbit muscle myosin ~215kDa. A dimer of SOD is occasionally observed at ~32kDa. The proteins were nitrated using peroxynitrite (Catalog # 20-107). See Balafanova, Z., et al., J. Biol. Chem. 277: 15021-15027.
      Product Information
      ApplicationNitrotyrosine Immunoblotting Control primarily used in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in western blot using anti-Nitrotyrosine (06-284 or 05-233).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size40 blots
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Nitrotyrosine Immunoblotting Control2958562
      Nitrotyrosine Immunoblotting Control - 24252552425255
      Nitrotyrosine Immunoblotting Control - 1724917249
      Nitrotyrosine Immunoblotting Control - 1842418424
      Nitrotyrosine Immunoblotting Control - 2097720977
      Nitrotyrosine Immunoblotting Control - 2123521235
      Nitrotyrosine Immunoblotting Control - 2326723267
      Nitrotyrosine Immunoblotting Control - 2370923709
      Nitrotyrosine Immunoblotting Control - 2804628046
      Nitrotyrosine Immunoblotting Control - DAM1768682DAM1768682


      Reference overviewPub Med ID
      Protein tyrosine nitration in the cell cycle.
      Min Jia,Claudia Mateoiu,Serhiy Souchelnytskyi
      Biochemical and biophysical research communications  413  2011

      Show Abstract
      21893039 21893039
      Changes in the mucosa of the Roux-limb after gastric bypass surgery.
      Emma Spak,Per Björklund,Herbert F Helander,Michael Vieth,Torsten Olbers,Anna Casselbrant,Hans Lönroth,Lars Fändriks
      Histopathology  57  2010

      Show Abstract
      21054493 21054493
      Medical schools in the United States, 2005-2006.
      Barbara Barzansky,Sylvia I Etzel
      JAMA : the journal of the American Medical Association  296  2006

      17370352 17370352
      Antibodies that recognize nitrotyrosine.
      Ye, Y Z, et al.
      Meth. Enzymol., 269: 201-9 (1996)  1996

      8791650 8791650
      Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry.
      Beckmann, J S, et al.
      Biol. Chem. Hoppe-Seyler, 375: 81-8 (1994)  1994

      8192861 8192861
      ALS, SOD and peroxynitrite.
      Beckman, J S, et al.
      Nature, 364: 584 (1993)  1993

      8350919 8350919
      Peroxynitrite-mediated tyrosine nitration catalyzed by superoxide dismutase.
      Ischiropoulos, H, et al.
      Arch. Biochem. Biophys., 298: 431-7 (1992)  1992

      Show Abstract
      1416974 1416974
      Nitrotyrosine as a new marker for endogenous nitrosation and nitration of proteins.
      Ohshima, H, et al.
      Food Chem. Toxicol., 28: 647-52 (1990)  1990

      Show Abstract
      2272563 2272563