509290 OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

509290
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      Overview

      Replacement Information

      Key Spec Table

      Empirical FormulaCAS #
      C₂₀H₄₀N₄O₁₀ · 2H₂SO₄108321-42-2

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      5.09290.0001
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          Glass bottle 500 mg
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                          Description
                          OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                          Catalogue Number509290
                          Brand Family Calbiochem®
                          SynonymsNMD Inhibitor I
                          References
                          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                          Product Information
                          CAS number108321-42-2
                          FormWhite powder
                          Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                          Applications
                          Biological Information
                          Biological activityAssay: ≥730 µg/mg
                          Purity≥90% by HPLC
                          Physicochemical Information
                          ContaminantsHeavy metals: ≤100 ppm
                          Dimensions
                          Materials Information
                          Toxicological Information
                          Safety Information according to GHS
                          RTECSCB9378500
                          Safety Information
                          Product Usage Statements
                          Storage and Shipping Information
                          Ship Code Ambient Temperature Only
                          Toxicity Standard Handling
                          Storage +15°C to +30°C
                          Do not freeze Ok to freeze
                          Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
                          Packaging Information
                          Transport Information
                          Supplemental Information
                          Specifications

                          Documentation

                          SDS

                          Title

                          Safety Data Sheet (SDS) 

                          References

                          Reference overview
                          Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                          Data Sheet

                          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                          Revision03-December-2013 JSW
                          SynonymsNMD Inhibitor I
                          DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                          FormWhite powder
                          Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
                          CAS number108321-42-2
                          RTECSCB9378500
                          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                          Purity≥90% by HPLC
                          ContaminantsHeavy metals: ≤100 ppm
                          Biological activityAssay: ≥730 µg/mg
                          Storage +15°C to +30°C
                          Do Not Freeze Ok to freeze
                          Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
                          Toxicity Standard Handling
                          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                          Jimenez, A., and Davies, J. 1980. Nature 287, 869.