524371 | PHM-L LIPOSORB™ Absorbent

524371
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Designed to selectively remove lipoproteins from plasma or serum by batch procedure or column chromatography; antibody content and coagulation factors remain intact. Is directly applicable with no further additions, affording improved serum or plasma stability. Has very low solubility in most solvents and has excellent chemical stability in acids and bases. PHM-L LIPOSORB™ Absorbent is resistant to enzymatic degradation and to activation of coagulation factors. Is heat stable and can be sterilized by autoclaving (120°C) without altering its binding capacity. Has high capacity with no volume limitation and is simple to use without expensive apparatus. Offers significant technical advantages over phenylagarose. One gram of absorbent is sufficient to remove lipids from 25 ml of serum or ascites.

      Capacity: >50 mg lipoprotein binding per g PHM-L LIPOSORB™ Absorbent.
      Catalogue Number524371
      Brand Family Calbiochem®
      References
      Product Information
      FormSolid
      FormulationPolyhydroxymethylene substituted by fat oxethylized alcohol.
      Applications
      Biological Information
      Primary Targetremoves lipids
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution store in the refrigerator (4°C). Solutions are stable for up to 3 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      524371
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-January-2011 RFH
      DescriptionAn absorbent designed to selectively remove lipids including lipoproteins from plasma, serum, or ascites. Antibody content and coagulation factors remain intact. It is applied with no further additions, thereby maintaining improved serum or plasma stability. It has very low solubility in most solvents and has excellent stability in acids and bases. Resistant to enzymatic degradation and to activation of coagulation factors. It is heat stable and can be sterilized by autoclaving (120°C) without altering its binding capacity. Has a high capacity with no volume limitation and is simple to use without expensive appartus. It offers significant technical advantages over phenylsepharose. May be used by batch procedure or by column chromatography (see below). Binding capacity: >50 mg lipoprotein binding per gram PHM-L LIPOSORB™ Absorbent (see label for lot-specific binding capacity); 1 g is sufficient to remove lipids from 25 ml serum or ascites. Swell volume: 10 ml/g PHM-L LIPOSORB™ Absorbent.
      FormSolid
      FormulationPolyhydroxymethylene substituted by fat oxethylized alcohol.
      Recommended reaction conditionsBatch Procedure: 1. Mix 1.5 parts plasma, serum, or ascites with 1 part equilibrated (see below) PHM-L LIPOSORB™ Absorbent in an appropriate tube and mix thoroughly (for fresh samples 30-60 s using a vortex is adequate). 2. Centrifuge for 10 min at 3000 rpm. 3. Transfer the top layer to a clean tube; this layer contains the clarified plasma, serum, or ascites. The lipids will remain in the bottom layer and can be discarded. Column Chromatography Procedure 1. Following equilibration (see below) it is helpful to briefly boil the absorbent (in a microwave) and let it cool and settle for a few minutes before packing the column. This results in the formation of larger aggregates, thereby increasing the column flow rate without compromising the binding capacity. 2. Pour the PHM-L LIPOSORB™ Absorbent into a suitable column that has previously been siliconized; allow to settle. 3. Apply 125 ml plasma, serum, or ascites to the column and allow it to pass through by gravity; do not apply pressure or vacuum. 4. Rinse the column with suitable buffer. If plasma is used the buffer should contain 10 mM sodium citrate. 5. The flow-through contains the clarified sample. Regeneration 1. Wash the absorbent with 20 mM sodium deoxycholate to elute all lipoproteins. 2. Wash with 100 mM NaOH to elute all remaining proteins. 3. Neutralize the column by washing several times with water.
      SolubilityReconstitute 1 g in 15 ml 150 mM sodium chloride or aqueous buffer, stir to mix, and allow to equilibrate and swell for 15 min.
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution store in the refrigerator (4°C). Solutions are stable for up to 3 months at 4°C.
      Toxicity Standard Handling