14-109 | PTP-1B Protein, Active, Agarose Conjugate, 50 µg

14-109
50 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-109
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPTP-1B Protein, Active, Agarose Conjugate, 50 µg
      OverviewDephosphorylates tyrosine-phosphorylated peptides and proteins
      References
      Product Information
      Applications
      ApplicationActive, agarose conjugate. Dephosphorylates tyrosine-phosphorylated peptides & proteins, for use in Phosphatase Assays.
      Key Applications
      • Phosphatase Assay
      Biological Information
      SourceGST-fusion to full-length human PTP-1B expressed in E. coli.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • PTPN1
      • PTP-1B
      • PTP1B
      Protein TargetPTP-1B
      Purification MethodGlutathione-agarose
      Target Sub-FamilyTyr phosphatase
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
      SIZE: 435 amino acids; 49967 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
      PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
      SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
      Molecular Weight76kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by measuring the hydrolysis of p-nitrophenyl phosphate (pNPP)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20 C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      PTP-1B, active, agarose conjugate - 16274 16274
      PTP-1B, active, agarose conjugate - 16699 16699
      PTP-1B, active, agarose conjugate - 18507 18507
      PTP-1B, active, agarose conjugate - 18714 18714
      PTP-1B, active, agarose conjugate - 18812 18812
      PTP-1B, active, agarose conjugate - 18831 18831
      PTP-1B, active, agarose conjugate - 19045 19045
      PTP-1B, active, agarose conjugate - 19677 19677
      PTP-1B, active, agarose conjugate - 20677 20677
      PTP-1B, active, agarose conjugate - 21842 21842

      References

      Reference overviewApplicationPub Med ID
      Insulin regulates the dynamic balance between Ras and Rap1 signaling by coordinating the assembly states of the Grb2-SOS and CrkII-C3G complexes
      Okada, S., et al
      Embo J, 17:2554-65 (1998)  1998

      Kinase Assay9564038 9564038
      Interferon gamma-dependent induction of human intercellular adhesion molecule-1 gene expression involves activation of a distinct STAT protein complex
      Naik, S. M., et al
      J Biol Chem, 272:1283-90 (1997)  1997

      Phosphatase Assay8995433 8995433
      Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinase
      Krek, W., et al
      Cell, 78:161-72 (1994)  1994

      Kinase Assay8033208 8033208
      Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response.
      Wakao, H, et al.
      EMBO J., 13: 2182-91 (1994)  1994

      Show Abstract
      Phosphatase Assay7514531 7514531
      The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence.
      Frangioni, J V, et al.
      Cell, 68: 545-60 (1992)  1992

      Show Abstract
      1739967 1739967

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      Alternative Format

      Catalogue Number Description  
      14-358 PTP-1B Protein, 500 units Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Phosphatases
      Life Science Research > Proteins and Enzymes > Purified Phosphatases