Pellicon 2 Microfiltration Cassettes

A new standard in cassette style tangential flow filtration devices.

Overview

Specifications

Ordering Information

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Mini · C Screen Clear Sorting & Filtering
Catalogue Numbericon Filter Codeicon Pore Sizeicon
P2DVPPC01DVPP 0.65 µm Show Pricing & Availability
P2GVPPC01GVPP 0.22 µm Show Pricing & Availability
P2HVMPC01HVMP 0.45 µm Show Pricing & Availability
P2VVPPC01VVPP 0.1 µm Show Pricing & Availability

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Mini · V Screen Clear Sorting & Filtering
Catalogue Numbericon Filter Codeicon Pore Sizeicon
P2DVPPV01DVPP 0.65 µm Show Pricing & Availability
P2GVPPV01GVPP 0.22 µm Show Pricing & Availability
P2HVMPV01HVMP 0.45 µm Show Pricing & Availability
P2VVPPV01VVPP 0.1 µm Show Pricing & Availability

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Cassette · C Screen Clear Sorting & Filtering
Catalogue Numbericon Filter Codeicon Pore Sizeicon
P2DVPPC05DVPP 0.65 µm Show Pricing & Availability
P2GVPPC05GVPP 0.22 µm Show Pricing & Availability
P2HVMPC05HVMP 0.45 µm Show Pricing & Availability
P2VVPPC05VVPP 0.1 µm Show Pricing & Availability

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Cassette · V Screen Clear Sorting & Filtering
Catalogue Numbericon Filter Codeicon Pore Sizeicon
P2DVPPV05DVPP 0.65 µm Show Pricing & Availability
P2GVPPV05GVPP 0.22 µm Show Pricing & Availability
P2HVMPV05HVMP 0.45 µm Show Pricing & Availability
P2VVPPV05VVPP 0.1 µm Show Pricing & Availability

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Maxicassette · C Screen Clear Sorting & Filtering
Catalogue Numbericon Filter Codeicon Pore Sizeicon
P2GVPPC25GVPP 0.22 µm Show Pricing & Availability
P2HVMPC25HVMP 0.45 µm Show Pricing & Availability
P2VVPPC25VVPP 0.1 µm Show Pricing & Availability

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Maxicassette · V Screen Clear Sorting & Filtering
Catalogue Numbericon Filter Codeicon Pore Sizeicon
P2DVPPV20DVPP 0.65 µm Show Pricing & Availability
P2GVPPV20GVPP 0.22 µm Show Pricing & Availability
P2HVMPV20HVMP 0.45 µm Show Pricing & Availability
P2VVPPV20VVPP 0.1 µm Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    Clarification and Recovery of Recombinant Proteins using a Cascade Tangential Flow Filtration (TFF) System

    Data Sheet

    Title
    Pellicon® 2 Filters and Holders

    References

    Reference overview
    Tetanus Toxoid Purification: A Case Study
    Vancetto, Mary Dalva Caparroz, Jose Marcos de Oliveira, Sally Muller Affonso Prado, Fernando Fratelli, and Hisako Gondo Higashi; Nature Biotechnology Volume 15
    Nature Biotechnology Volume 15  1997

    Evaluation of Microporous Tangential-Flow Filtration in the Production of Diphtheria and Pertussis Vaccines
    Rao, Y. Udaya Bhaskara, M.S. Mahadevan, and Stephen L. Michaels; Pharmaceutical Technology
    Pharmaceutical Technology  1992

    Validation of Tangential Flow Filtration (TFF) Systems
    Michaels, Stephen L.
    Journal of Parenteral Science and Technology (Sept/Oct 1991).  1991

    FAQ

    QuestionAnswer
    What is Normalized Water Permeability?NWP is a measure of the ability of pure water to pass through the filter membrane. NWP is a property of the TFF system and is contributed to by the membrane as well as the feed and permeate flow paths in the filter.
    How is NWP measured?NWP is measured by filtering pure water throught he system at controlled cross flow, Trans Membrane Pressure and temperature. The filtrate flow rate is measured. The flow rate is normalized to the membrane area, TMP and temperature.

    Please refer to the operator's manual or contact Techical Service for further questions regarding the calculation of NWP.
    How does NWP aid in effective cleaning procedure development?Fouling and polarization leave residues on the membrane that can reduce filter permeability. Effective cleaning removes these residues, restoring the permeability of the membrane. Ideally, NWP after cleaning should equal NWP before use although there can be variation up to +/-15% due to membrane compaction under pressure and measurement error.

    NWP validates the ability of cleaning to restore filtration rate of a system.
    What is the hold-up volume of a Pellicon 2 Mini cassette?The approximate hold-up volume for a Pellicon 2 mini cassette (0.1m2) is 18 ml upstream and 10 ml downstream.
    What is the hold up volume for a 0.5m2 Pellicon 2 cassette?The approximate hold up volume is 90 ml on the feed side and 50 ml on the permeate side.
    What is the hold-up volume for a Pellicon 2 Maxi cassette (2.5m2)?The approximate hold up volume for the Pellicon 2 Maxi cassettes is 450 ml on the feed side and 250 ml on the permeate side.
    Are there any microporous offerings in the tangential flow filtration line of products?While the Prep/Scale and Pellicon XL are not available in a microporous format, the Pellicon II Mini and Pellicon II systems are. The microporous membranes are made of Millipore's Durapore membrane, low protein binding PVDF, and are available in 0.1um, 0.22um, 0.45um, and 0.65um.
    Can the Pellicon II cassettes be used with older Pellicon holder?Yes, the new Pellicon II cassettes may be used the old Pellicon I holder. They will fit for operation without any adaptation.
    How many cassettes can fit into the Pellicon II Mini holder at one time?The Pellicon II Mini holder can hold from one to three of the Mini cassettes at one time. As a general rule of thumb, for every 10 L of process volume, add a cassette to the system.
    What is the length of the retentate and permeate channel in the Pellicon platform cassettes?The length of the retentate and permeate channel is 15.5 cm.

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    Categories

    Biopharmaceutical Manufacturing > Downstream Processing > Ultrafiltration > Pellicon Cassettes > Pellicon 2 Cassettes