|Pellicon® 2 Filters and Holders|
|Tetanus Toxoid Purification: A Case Study|
Vancetto, Mary Dalva Caparroz, Jose Marcos de Oliveira, Sally Muller Affonso Prado, Fernando Fratelli, and Hisako Gondo Higashi; Nature Biotechnology Volume 15
Nature Biotechnology Volume 15 1997
|What is the minimum working volume for the Pellicon system?||Depending on the length of tubing involved and the set up of the system, it is possible to bring the working volume down to 150 mL for the standard holder. With the low retentate holder (catalog number XX42PRV60, acrylic), it is possible to bring the working volume down to 50 mL.|
|What are turbulence promoters?||Turbulence promoters are screens that are inserted into the feed and/or filtrate channels in TFF modules in order to increase the "turbulence" in the channels of the module and to reduce the occurence of concentration polarization (the gel layer that can form on membranes therefore slowing flux). These turbulence promoted channels allow for higher fluxes with lower pumping requirements which makes them more efficient than open channels without turbulence promoters.|
|Is there a prefiltration requirement for use with the Pellicon system?||Yes, the Pellicon system requires a 100um prefiltration. Depending on the process volume, Millipore offers an array of prefiltration modules. Contact Technical Services for help in choosing a module.|
|How many cassettes (0.5m2 or 2.5 m2 Maxi) can you stack into the Pellicon II holder?||The systems are shipped with long tie rods (catalog number XX42P0L64 for acrylic holders and XX42P0L62 for stainless steel holders) you may stack up to 10 cassettes of 0.5 m2 each, or two Maxi cassettes. If you have the short tie rods, you may stack up to 5 cassettes of 0.5 m2 each for a total area of 2.5 m2, or one Maxi cassette of 2.5 m2.|
Pellicon 2 Microfiltration CassettesA new standard in cassette style tangential flow filtration devices.
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Pellicon® 3 CassettesAdvanced, high performance cassettes ideally suited to today's demanding filtration processes that require more rigorous operating pressures, temperatures and caustic cleaning regimes
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Pellicon® 2 Ultrafiltration CassettesDesigned with process scale-up and applications development in mind.
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This product is perfect for anyone who needs a benchtop cassette holder versatile enough to support either microporous or ultrafiltration tangential flow filtration.
- Linear Scale-up – a single Pellicon® acrylic holder has up to 10 times the linear scale-up potential in filter area (Note: increased pump capacity is required for scale-up).
- High Product Recoveries – due to low hold-up volumes.
- Utilizing Existing Equipment – all equipment is included to connect to your existing peristaltic tubing pump (pump not included). An interchangeable selection of fittings and pumps is also available for changing system capabilities.
- Production Range – depending upon your needs, between 1 and 500 liter batches can be filtered – in either a matter of minutes or a few hours.
For ordering information on pumps offered separately, please refer to “Accessories and Spare Parts for Pellicon Systems, Holders and Assemblies."
|Holder and Assembly Capacity (filter area)|
|Standard Holder:||0.5 – 2.5 m² (5.5 – 27 ft² ) as shipped Expandable to 5 m² (54 m² ) with long tie rods Up to 500 liter batches with long tie rods|
|Low Retentate Volume Holder:||0.5 m² (5.5 ft² ) as shipped. Assembly capacity depends upon the type of pump tubing size.|
|Holders:||Pellicon® cassette acrylic holder with ¾ in. sanitary clamp adapters, clamps, and gaskets. They are supplied with a socket wrench and operating manual.|
|Assemblies:||All assemblies include holder, two glycerine-filled stainless steel pressure gauges (0 – 7 bar, 0 – 100 psi dual scale), permeate ball valve, holder-to-tubing adapter fittings, clamps and gaskets, quick-assembly instructions and operating manual.|
|Materials of Construction|
|Wetted Surfaces:||Acrylic manifold with polypropylene adapter fittings and silicone sanitary gaskets|
|Non-wetted Surfaces:||Nylon-coated aluminum pressure plates; 316 stainless steel tie rods and washers; nylon-encapsulated 301 stainless steel holder compression assemblies; silicone bronze nuts|
|Inlet/Outlet:||¾ in. sanitary clamp adapters|
|Gauge Ports:||¼ in. NPTF|
|Feed/Retentate Tubing:||6.4 mm (¼ in.)|
|Permeate Tubing:||6.4 mm (¼ in.) tubing hose barb ball valve assembly|
|Operating Temperature Range||4 – 50 °C|
|Maximum Inlet Pressure|
|Holder:||7 bar (100 psi)|
|Tubing:||2.8 bar (40 psi) intermittent, 1.4 bar (20 psi) for 40 h|
|Recommended Feed Flow||4 – 33 L/min/m² of installed filter area|
|Minimum Retentate Working Volume|
|Standard Holder:||120 mL|
|Low Retentate Holder:||50 mL|
|* With 0.5 m² area filter and 1.2 m of 6.4 mm (¼ in.) I.D. tubing|