ECM504 | QCM Chemotaxis Cell Migration Assay, 24-well (3µm), colorimetric

ECM504
1 plate  24 wells
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      ACTChromogenic
      Description
      Catalogue NumberECM504
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM Chemotaxis Cell Migration Assay, 24-well (3µm), colorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). One such process, leukocyte extravasation, is crucial for appropriate and effective immune response. Neutrophils normally exist in a resting state as they circulate though the body. However, upon interaction with small molecules known as chemoattractants, they rapidly respond with endothelial adhesion followed by emigration from the vasculature and chemotaxis to the site of inflammation. These chemoattractant receptors activate heterotrimeric GTP-binding proteins (G proteins) that initiate numerous elaborate signal transduction cascades, culminating in neutrophil migration and activation. Once at the site of inflammation, neutrophils respond with phagocytosis, superoxide generation, and the release of degradative enzymes.
      Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted.
      Most migration assays utilize an 8 µm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Millipore 3 µm QCM™ Chemotaxis Assay 24-well- colorimetric utilizes a 3 µm pore size, which is appropriate for leukocyte migration. Cells migrated toward chemoattractants were measured by WST-1 (cell viability stain), and quantitated by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, and chemokinesis.
      The Millipore QCM™ 3 µm Chemotaxis Assay 24-well-colorimetric provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
      • QCM™ 8µm 24-well Chemotaxis Cell Migration Assays (ECM508, 509)
      • QCM™ 5µm 24-well Chemotaxis Cell Migration Assay (ECM507)
      • QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
      • QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
      • QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
      • QCM™ 96-well ECMatrix™ Cell Invasion Assay (ECM555)
      • QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)
      Materials Required but Not Delivered1. 96-well ELISA plate.
      2. Precision pipettes: sufficient for aliquoting cells.
      3. Harvesting buffer: EDTA or trypsin based cell detachment buffer, or other cell detachment formulations as optimized by individual investigators. Millipore's ready-to-use non-mammalian detachment solution, Accutase™ (Cat. No. SCR005) can also be used.
      Note: Trypsin cell detachment buffer maybe required for strongly adherent cell lines, but can strip cell surface proteins. Allow sufficient time for cell receptor recovery.
      4. Tissue culture growth medium appropriate for subject cells, such as RPMI-1640 containing 10% FBS.
      5. Chemoattractants (eg. 10% FBS) or pharmacological agents for addition to culture medium, if screening is desired.
      6. Sterile PBS or HBSS to wash cells.
      7. Distilled water.
      8. Low speed centrifuge and tubes for cell harvesting.
      9. CO2 incubator appropriate for subject cells.
      10. Hemocytometer or other means of counting cells.
      11. Trypan blue or equivalent viability stain.
      12. Spectrophotometer (microplate reader).
      13. Sterile cell culture hood.
      References
      Product Information
      Components
      • Sterile 3 µm 24-well Cell Migration Plate Assembly: (Part No. 2005709) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
      • WST-1 Reagent: (Part No. CS201370) One vial.
      • Electro Coupling Solution: (Part No. CS201382) 5 ml.
      • Forceps: (Part No. 10203) One each.
      Detection methodChromogenic
      Applications
      ApplicationThis 3 um QCM Chemotaxis Assay 24-well plate -colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.
      Key Applications
      • Activity Assay
      Application NotesThe Millipore 3 µm QCM™ Chemotaxis Assay 24-well-colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle. The 3 µm pore size of this assay's Boyden chambers is appropriate for studying leukocyte migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples.

      The Millipore 3 µm QCM™ Chemotaxis Assay 24-well-colorimetric is intended for research use only; not for diagnostic applications.
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe two Sterile 3 µm 24-well Cell migration plate assembly and forceps are stored at 2°-8°C. WST-1 reagent and Electro Coupling Solution are stored at -20°C.
      Packaging Information
      Material Size1 plate
      Material Package24 wells
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays