ECM507 | QCM Chemotaxis Cell Migration Assay, 24-well (5 µm), fluorimetric

ECM507
1 kit  Contains 2 plates sufficient for 24 assays
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      ACTFluorescent
      Description
      Catalogue NumberECM507
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM Chemotaxis Cell Migration Assay, 24-well (5 µm), fluorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      The CHEMICON® QCM™ Chemotaxis 5 μm 24-well Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/macrophage migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples.

      Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammationIn immune responses, monocyte migration is critical for providing immunological defense and eliminating infectious agents. Monocytes (macrophage precursors) circulate the blood until encountering specific chemotactic factors that initiate the migration process. Once engaged, monocytic cells transverse the vascular endothelium in a multi-step mechanism by: A) E-selectin mediated monocyte rolling B) cell adhesion C) transmigration into the subendothelium. During this migration and entering the tissue, monocytes develop/differentiate into mature macrophages, increasing in size, phagocytic activity, and proteolytic enzyme secretion. Once at the site of infection, mature macrophage respond to infection by phagocytosis, cytokine secretion, respiratory burst, T-cell antigen presentation, and digestive enzyme secretion.

      Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the topside of insert, manual staining and counting. Recently, a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

      The Chemicon QCM™ Chemotaxis 5 μm 24-well Migration Assay does not require cell labeling, scraping, washing, or counting. The 24-well inserts and homogenous fluorescence detection format allow for convenient screening and quantitative comparison of multiple samples.

      In the Chemicon QCM™ Chemotaxis 5 μm 24-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with the Cell Detachment Solution. These cells are subsequently lysed and detected by the patented CyQUANT® GR dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids.

      Most migration assays utilize an 8 μm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Chemicon QCM™ Chemotaxis 5 μm 24-well Migration Assay utilizes a 5 μm pore size, which is appropriate for monocyte/macrophage migration. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.

      The Chemicon QCM™ Chemotaxis 5μm 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
      References
      Product Information
      Components
      • Sterile 5 μm 24-well Cell Migration Plate Assembly: (Part No. 2005708)
      • Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
      • Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
      • 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
      • CyQUANT® GR Dye: (Part No. 90132) One vial - 75 μL
      • Forceps: (Part No. 10203) One each.
      Detection methodFluorescent
      Applications
      ApplicationThe QCM Chemotaxis 5 um 24-well Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit components at 2° to 8°C, up to the expiration date provided on the kit.
      Packaging Information
      Material Size1 kit
      Material PackageContains 2 plates sufficient for 24 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Distinct signaling pathways for MCP-1-dependent integrin activation and chemotaxis
      Ashida, N, et al
      J Biol Chem, 276:16555-16560 (2001)  2001

      11278464 11278464
      Transmembrane motility assay of transiently transfected cells by fluorescent cell counting and luciferase measurement.
      Gildea, J J, et al.
      BioTechniques, 29: 81-6 (2000)  2000

      Show Abstract
      10907081 10907081
      Wound healing--aiming for perfect skin regeneration.
      Martin, P
      Science, 276: 75-81 (1997)  1997

      Show Abstract
      9082989 9082989
      Transient functional expression of alphaVbeta 3 on vascular cells during wound repair.
      Clark, R A, et al.
      Am. J. Pathol., 148: 1407-21 (1996)  1996

      Show Abstract
      8623913 8623913
      Simultaneous detection of free radical release and membrane current during phagocytosis
      Holevinsky, K O and Nelson, D J
      J Biol Chem, 270:8328-8336 (1995)  1995

      7713941 7713941

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
      Cell Migration and Invasion: Choosing the Right Assay
      EpiGRO™, EndoGRO™ and FibroGRO™ Reagents

      Data Sheet

      Title
      QCM Chemotaxis (5 μm) 24-well Cell Migration Assay, Fluorimetric - Data Sheet
      QCM Chemotaxis 5μm 24-well Cell Migration Assay, Fluorimetric - Data Sheet

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      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays