ECM512 | QCM Chemotaxis Cell Migration Assay, 96-well (5 µm), fluorimetric

ECM512
1 plate  96 wells
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      ACTFluorescent
      Description
      Catalogue NumberECM512
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM Chemotaxis Cell Migration Assay, 96-well (5 µm), fluorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (Clark et al., 1996; Martin, 1997). In immune responses, monocyte migration is critical for providing immunological defense and eliminating infectious agents. Monocytes (macrophage precursors) circulate the blood until encountering specific chemotactic factors that initiate the migration process. Once engaged, monocytic cells transverse the vascular endothelium in a multistep mechanism by: A) E-selectin mediated monocyte rolling B) cell adhesion C) transmigration into the subendothelium (Ashida et al., 2001). During this migration and entering the tissue, monocytes develop/differentiate into mature macrophages, increasing in size, phagocytic activity, and proteolytic enzyme secretion. Once at the site of infection, mature macrophage respond to infection by phagocytosis, cytokine secretion, respiratory burst, T-cell antigen presentation, and digestive enzyme secretion (Holevinsky & Nelson, 1995).

      Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the topside of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

      The Chemicon QCM™ 96-well 5 μm Migration Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples (Gildea et al., 2000).

      In the Chemicon QCM™ 5 μm 96-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR® dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (Jones et al., 2001).

      Most migration assays utilize an 8 μm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Chemicon QCM™ 5 μm 96-well Migration Assay utilizes a 5 μm pore size, which is appropriate for studying monocyte/macrophage migration. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.

      The Chemicon QCM™ 5 μm 96-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.

      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

    • QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
    • QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
    • QCM™ 96-well Cell Invasion Assay (ECM555)
    • 24-well Insert Cell Migration and Invasion Assay Systems
    • CytoMatrix™ Cell Adhesion strips (ECM protein coated)
    • QuantiMatrix™ ECM protein ELISA kits

      Application

      The Chemicon QCM™ 96-well 5μm Migration Assay is ideal for studying chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/ macrophage migration. Each kit provides sufficient materials for the evaluation of 96 samples.

      The Chemicon QCM™ 5μm 96-well Migration Assay is intended for research use only; not for diagnostic applications.
    • References
      Product Information
      Detection methodFluorescent
      Applications
      ApplicationThe QCM 5 um 96-well Migration Assay utilizes a 5 um pore size, which is appropriate for studying monocyte/macrophage migration.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Toxicity and intraocular properties of a novel long-acting anti-proliferative and anti-angiogenic compound IMS2186.
      Iryna A Falkenstein,Lingyun Cheng,Flossie Wong-Staal,Ajay M Tammewar,Erin C Barron,Gabriel A Silva,Qi-Xiang Li,Dehua Yu,Michelle Hysell,Guohong Liu,Ning Ke,James E Macdonald,William R Freeman
      Current eye research  33  2008

      Show Abstract Full Text Article
      18600493 18600493

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
      Cell Migration and Invasion: Choosing the Right Assay
      EpiGRO™, EndoGRO™ and FibroGRO™ Reagents

      Data Sheet

      Title
      QCM™ Chemotaxis 5μm 96-Well Cell Migration Assay Data Sheet
      QCM™ Chemotaxis 5μm 96-Well Cell Migration Assay- Data Sheet

      Related Products & Applications

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays