03-112 | RIPAb+ QKI-5

03-112
10 assays  10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey Applications
      H, M, RRIP, IP, WB
      Description
      Catalogue Number03-112
      Brand Family Upstate
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+ QKI-5
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      Alternate Names
      • Quaking Protein
      Background InformationQKI is an RNA-binding protein that plays a central role in myelinization. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. It is regulated by alternative splicing.
      References
      Product Information
      FormatPurified
      Control
      • Includes negative control rabbit IgG antibody and control primers specific for human MAGI1.
      PresentationAnti-QKI-5 (Rabbit Polyclonal). One vial containing 50 μL of rabbit serum. Store at -20°C.

      Normal Rabbit IgG. One vial containing 125 μg Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.

      RIP Primers, MAGI1. One vial containing 75 μL of 5 μM of each primer specific for human MAGI1. Store at -20°C.
      FOR: TAT TCC AAA CCA CCC TCC AA
      REV: CAA CTC AGA AGG GTT TCG TGT
      Applications
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation from RIP lysate:
      Representative lot data.
      RIP lysate from HeLa cells (2 X 106 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-QKI-5 antibody (Please see figures).

      Automated Microfluidics-based Electrophoretic RNA Separation and Analysis:
      RIP Lysate prepared from NTera-2 cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-QKI-5 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of QKI-5-associated RNA was verified by automated microfluidics-based electrophoretic RNA separation and analysis.
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

      Electropherograms were generated by plotting fluorescence intensities versus migration times (Please see figures).
      A virtual gel view was created from this data (Please see figures).

      Western Blot Analysis: 1:500-1:1,000 dilution of a previous lot detected QKI-5 in western blot.
      Biological Information
      ImmunogenSynthetic peptide corresponding to mouse QKI-5.
      HostRabbit
      SpecificityRecognizes QKI-5 (Quaking Protein).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryQKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998 [PubMed 9671495]).[supplied by OMIM]
      Gene Symbol
      • QKI
      • DKFZp586I0923
      • Hqk
      • QK3
      • QK
      • HKQ
      • HqkI
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96PU8 # RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor (By similarity).
      SIZE: 341 amino acids; 37671 Da
      SUBUNIT: Homodimer. Does not require RNA to homodimerize. Able to heterodimerize with BICC1 (By similarity).
      SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity).
      TISSUE SPECIFICITY: Expressed in the frontal cortex of brain.
      PTM: Methylated by PRMT1 (By similarity). & Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA (By similarity).
      DISEASE: SwissProt: Q96PU8 # May play a role in genetic susceptibility to schizophrenia. QKI levels of some isoforms such as isoform 6, are frequently down-regulated in schizophrenic patients. & Deletion of the QKI gene may play a role in astrocytic tumors.
      SIMILARITY: SwissProt: Q96PU8 ## Contains 1 KH domain.
      Molecular Weight~40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      RIP Lysate prepared from SHSH-5Y cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-QKI-5 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of QKI-5-associated RNA was verified by qPCR using RIP Primers MAGI1 (Please see figures).
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies