03-179 | RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set

03-179
10 assays  10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey Applications
      HRIP, WB, IP
      Description
      Catalogue Number03-179
      Brand Family Upstate
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
      Alternate Names
      • ChET 9 protein
      • Chromatin precipitated E2F target 9 protein
      • Joined to JAZF1 protein
      • Suppressor of zeste 12 protein homolog
      • joined to JAZF1
      • suppressor of zeste 12 homolog (Drosophila)
      Background InformationSUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
      References
      Product Information
      FormatPurified
      Control
      • Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human lincRNA SFPQ.
      PresentationAnti-SUZ12 (Mouse Monoclonal) -
      One vial containing 50 μg of protein A purified antibody in PBS containing 0.1% azide, before the addition of 30% glycerol. Store at -20°C.

      Normal Mouse IgG -
      One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.

      RIP Primers, lincSFPQ -
      One vial containing 75 μL of 5 μM of each primer specific for the human lincRNA SFPQ. Store at -20°C.
      FOR: TCG TAC TGT TAG GCC CTT GG
      REV: AAC CTT GCA TGA AGA GCA CC
      Applications
      ApplicationThis RIPAb+ SUZ12 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation from RIP lysate:
      Representative lot data.
      RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG (Cat. #CS200621), or 5 µg of Anti-SUZ12 antibody (Cat. # CS203201).
      20 percent of the precipitated proteins (lane 1: mouse IgG, lane 2: SUZ12) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SUZ12 antibody (Cat. # CS203201, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232)
      Arrow indicates SUZ12 (Figure 2).

      Western Blot Analysis:
      Representative lot data.
      Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution).
      Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
      Arrow SUZ12 (~95 kDa) (Figure 3).
      Biological Information
      ImmunogenFull length, recombinant human SUZ12.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySUZ12
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. The specific function of this gene has not yet been determined.
      Gene Symbol
      • SUZ12
      • KIAA0160
      • CHET9
      • JJAZ1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15022 # Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
      SIZE: 739 amino acids; 83055 Da
      SUBUNIT: Component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like BMI1, CBX4, CBX8, PHC2, PHC3, SCMH1, RING1 and RNF2.
      SUBCELLULAR LOCATION: Nucleus (Probable).
      DISEASE: SwissProt: Q15022 # A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
      SIMILARITY: SwissProt: Q15022 ## Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. & Contains 1 C2H2-type zinc finger.
      Molecular Weight95 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG , or 5 µg of Anti-SUZ12 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of SUZ12-associated RNA was verified by qPCR using RIP Primers lincSFPQ, (Figure 1)
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RIPAb+ SUZ12 - 2037609 2037609
      RIPAb+ SUZ12 - 2080878 2080878
      RIPAb+ SUZ12 - NRG1860695 NRG1860695
      RIPAb+™ SUZ12 2468127
      RIPAb+™ SUZ12 -2650795 2650795
      RIPAb+™ SUZ12 -2697389 2697389
      RIPAb+™ SUZ12 Monoclonal Antibody/Primer Set 2953948
      RIPAb+™ SUZ12 Monoclonal Antibody/Primer Set 2952861
      RIPAb+™ SUZ12, Monoclonal Antibody/Primer Set 2836674
      RIPAb+™ SUZ12 - 2460969 2460969

      References

      Reference overviewApplicationPub Med ID
      The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation.
      Beckedorff, FC; Ayupe, AC; Crocci-Souza, R; Amaral, MS; Nakaya, HI; Soltys, DT; Menck, CF; Reis, EM; Verjovski-Almeida, S
      PLoS Genet  9  e1003705  2013

      Show Abstract
      Western Blotting23990798 23990798

      Posters

      Title
      Effective Nuclear RIP Protocols to Study Interactions between Proteins and Non-Coding RNAs

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies