AP512F | Sheep Anti-Human IgM, (Fc), FITC conjugate Antibody

500 µg  
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      Replacement Information
      Catalogue NumberAP512F
      Trade Name
      • Chemicon
      DescriptionSheep Anti-Human IgM, (Fc), FITC conjugate Antibody
      Alternate Names
      • Ig mu chain C region
      Background InformationImmunoglobin M (IgM) antibody is prominent in early immune responses to most antigens and is the predominant response in certain antibody responses such as "natural" blood group antibodies. IgM is the major immunoglobin expressed on surface B lymphocytes. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM.
      Product Information
      • Human hybridoma expressing EBV
      PresentationPurified sheep polyclonal in PBS containing 0.05% sodium azide and 15 mg/mL BSA. Depleted against rabbit IgM and human IgG.
      ApplicationDetect Human IgM using this Sheep anti-Human IgM, (Fc), FITC conjugate Antibody validated for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenHuman anti-Human IgM (myeloma) Fc5u fragment
      EpitopeIgM, (Fc)
      Concentration1 mg/mL
      SpecificityThis antibody reacts with human IgM, (Fc) as demonstrated by Flow Cytometry.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. No reactivity with IgG or IgA. May react with IgM of other species with low specificity.
      Antibody TypePolyclonal Antibody
      Purification MethodDepletion
      UniProt SummaryFUNCTION: IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as an homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts.

      SUBUNIT STRUCTURE: Immunoglobulin (Ig) molecules consist of two chains, one heavy chain (which can be alpha, delta, epsilon, gamma or mu) and one light chain (which can be kappa or lambda) each consisting of a variable and a constant region. An IgM molecule contains thus a mu heavy chain combined with either a kappa or a lambda light chains. It is found almost exclusively as an homopentamer in the serum. Membrane-bound IgM molecules are non-covalently associated with heterodimer of CD79A and CD79B.

      SUBCELLULAR LOCATION: Isoform 1: Secreted. Note: During differentiation, B-lymphocytes switch from expression of membrane-bound IgM to secretion of IgM.

      Isoform 2: Cell membrane; Single-pass type I membrane protein.

      POLYMORPHISM: All 4 combinations of the S/G and V/G polymorphisms at positions 191 and 215 have been observed in human mu chains.

      INVOLVEMENT IN DISEASE: Defects in IGHM are the cause of agammaglobulinemia type 1 (AGM1) [MIM:601495]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Ref.10

      SEQUENCE CAUTION: The sequence CAA33071.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

      The sequence CAA34971.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
      Molecular Weight50 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in human hybridoma expressing EBV.

      Flow Cytometry Analysis: 1:4,000 dilution of this antibody detected Sheep Anti-Human IgM, (Fc) in human hybridoma expressing EBV.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquotes for up to 6 months from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information