AQ300F | Sheep Anti-Mouse Ig Antibody, F(ab')2, FITC conjugate

200 µg  
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      Replacement Information
      Catalogue NumberAQ300F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionSheep Anti-Mouse Ig Antibody, F(ab')2, FITC conjugate
      OverviewThe reagent is an anti-mouse Ig antibody, F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin digestion and conjugated with 5(6)- carboxy-fluorescein-N-hydroxysuccinimide ester (fluorescein) and stabilized in buffer.The molar ratio of fluorescein: protein is between 3-4.

      PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.
      Product Information
      PresentationLyophilized. Buffer = 0.02 M sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.1% sodium azide


      Reconstitute with 200 µL of sterile distilled water.
      ApplicationSheep anti-Mouse Ig Antibody, F(ab')2, FITC conjugate is an antibody against Mouse Ig for use in IF.
      Key Applications
      • Immunofluorescence
      Application NotesThe reagent allows the detection of primary mouse antibodies (monoclonal/ polyclonal) in immunohistochemistry (Volson 1984), immunocytochemistry (Gonatas et al. 1987) as well as the detection of mouse monoclonal antibodies from hybridoma supernatants by indirect immunofluorescence.


      Immunohisto/cytochemistry: 1:50 (20 μg/mL)

      The concentrated stock solution (1.0 mg/mL) should be diluted with PBS, pH 7.1 with 15 mg/mL bovine serum albumin.
      Biological Information
      SpecificityThe reagent recognizes all mouse immunoglobulin classes and subclasses, independently from the type of the light chain. The antibody shows no cross- reactivity (<0.01%) with fetal calf serum (FCS) and normal serum from bovine, rabbit, goat, horse, and human. Weak reaction can be detected with normal serum from guinea pig (<0.1%). Cross-reactivity can be detected with normal serum from rat (<10%).


      Between 3 and 4.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen protected from light, the lyophilized antibody-conjugate is stable at +4°C.The reconstituted antibody conjugate solution is stable for 2 months at +4°C, when protected from light.The solution should be stored in aliquots at -20°C; repeated freezing and thawing should be avoided.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information



      Reference overviewPub Med ID
      Detection of plasma cell immunoglobulins in tissue sections optimally fixed for ultrastructural immunocytochemistry.
      Gonatas, N K, et al.
      J. Histochem. Cytochem., 35: 189-96 (1987)  1987

      Show Abstract
      3098833 3098833
      A biotin-avidin technique for the localisation of membrane-bound monoclonal antibodies by low power transmission electron microscopy.
      Volsen, S G
      J. Immunol. Methods, 72: 119-26 (1984)  1984

      Show Abstract
      6379052 6379052

      Data Sheet

      Sheep anti-Mouse Ig, (H+L) Fluorescein (FITC)-Conjugated Affinity Purified F(ab')2 - Data Sheet

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      Life Science Research > Antibodies and Assays > Secondary Antibodies