AQ301F | Sheep Anti-Rabbit IgG Antibody, F(ab')2, FITC conjugate

200 µg  
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      Replacement Information
      Catalogue NumberAQ301F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionSheep Anti-Rabbit IgG Antibody, F(ab')2, FITC conjugate
      OverviewThe reagent is an anti-rabbit antibody F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin cleavage. The fragments are coupled 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (fluorescein) and stabilized in buffer.The molar ratio of fluorescein/ protein is between 3 - 4.

      PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.
      Product Information
      PresentationLyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.1% sodium azide.


      Reconstitute with 1 mL of sterile distilled water.
      ApplicationSheep anti-Rabbit IgG Antibody, F(ab')2, FITC conjugate detects level of Rabbit IgG & has been published & validated for use in IF.
      Key Applications
      • Immunofluorescence
      Application NotesThe reagent allows a reliable and sensitive detection of rabbit IgG in immunohistochemistry and immunocytochemistry techniques. In addition, the absence of the "sticky" Fc portion avoids unspecific binding. Thus, the reagent is particularly suitable for use on sections and cells.


      20-50 μg/mL

      The concentrated stock solution (200 μg/mL) should be diluted with PBS, pH 7.1 containing 15 mg/mL BSA.

      Optimal working concentrations must be determined by the end user.
      Biological Information
      SpecificityThe reaction profile of the antibody was examined in an ELISA system: micro titer plates were coated with antigen and potentially cross-reacting IgG of other species in identical concentrations, incubated with anti-rabbit IgG-biotin and detected with a streptavidin-enzyme conjugate. In this system the antibody shows the following reaction profile: No cross-reactivity (<0.01%) with Ig from human, pig, bovine, goat, mouse and fetal calf serum (FCS). Weak reactivity (<1%) with Ig from horse and cross-reactivity (<5%) with Ig from rat and guinea pig.


      Between 3 and 4.
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored protected from light, the lyophilized antibody-conjugate is stable at 2-8°C.When stored protected from light the reconstituted antibody conjugate solution is stable for 2 months at 2-8°CThe reconstituted solution should be stored in aliquots at -20°C; repeated freezing and thawing should be avoided.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information



      Reference overviewPub Med ID
      Detection of plasma cell immunoglobulins in tissue sections optimally fixed for ultrastructural immunocytochemistry.
      Gonatas, N K, et al.
      J. Histochem. Cytochem., 35: 189-96 (1987)  1987

      Show Abstract
      3098833 3098833

      Data Sheet

      Sheep anti-Rabbit IgG, (H+L) Fluorescein (FITC)-Conjugated Affinity Purified F(ab')2 - Data Sheet

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      Life Science Research > Antibodies and Assays > Secondary Antibodies