AQ301R | Sheep Anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate

AQ301R
200 µg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAQ301R
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionSheep Anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate
      OverviewThe reagent is an anti-rabbit antibody F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin cleavage. The fragments are coupled to 5(6)-carboxy-rhodamine 101-N-hydroxysuccinimide ester (rhodamine) and stabilized in buffer.The molar ratio of rhodamine: protein is between 2-3.

      PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.
      References
      Product Information
      FormatRhodamine (TRITC)
      PresentationLyophilized from 0.02M Sodium phosphate, 0.25M NaCl, pH 7.1 with 15 mg/mL BSA and 0.1% sodium azide.

      RECONSTITUTION:

      Reconstitute with 1 mL of sterile distilled water.
      Applications
      ApplicationSheep anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate is an antibody against Rabbit IgG for use in IF.
      Key Applications
      • Immunofluorescence
      Application NotesThe reagent allows a reliable and sensitive detection of rabbit IgG immunohistochemistry, respectively immunocytochemistry, especially when interference of immunoglobulins of other species than rabbit may occur. In addition, the absence of the "sticky" Fc portion avoids unspecific binding. Thus, the reagent is particularly suitable for use on sections and cells.

      RECOMMENDED CONCENTRATION:

      20-50 μg/mL. It is recommended that the antibody be diluted with PBS, pH 7.1 containing 15 mg/mL BSA.

      Optimal working concentrations must be determined by the end user.
      Biological Information
      HostSheep
      SpecificityThe reaction profile of the antibody was examined in an ELISA system: micro titer plates were coated with antigen and potentially cross-reacting IgG of other species in identical concentrations, incubated with anti-rabbit IgG-biotin and detected with a streptavidin-enzyme conjugate. In this system the antibody shows the following reaction profile: No cross-reactivity (<0.01%) with Ig from human, porcine, bovine, goat, mouse and fetal calf serum (FCS). Weak reactivity (<1%) with Ig from horse and cross-reactivity (<5%) with Ig from rat and guinea pig.

      WAVE LENGTH:

      Absorption peak = 550 nm, Emission peak, 570 nm.
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored protected from light, the lyophilized antibody-conjugate is stable at 2-8°C.When stored protected from light the reconstituted antibody conjugate solution is stable for 2 months at 2-8°CThe solution should be stored in aliquots at -20°C.Repeated freezing and thawing should be avoided.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Destabilized adhesion in the gastric proliferative zone and c-Src kinase activation mark the development of early diffuse gastric cancer.
      Humar, B; Fukuzawa, R; Blair, V; Dunbier, A; More, H; Charlton, A; Yang, HK; Kim, WH; Reeve, AE; Martin, I; Guilford, P
      Cancer research  67  2480-9  2007

      Show Abstract
      17363565 17363565
      Cardiomyocyte cell cycle activation ameliorates fibrosis in the atrium.
      Nakajima, H; Nakajima, HO; Dembowsky, K; Pasumarthi, KB; Field, LJ
      Circulation research  98  141-8  2006

      Show Abstract Full Text Article
      16306446 16306446
      A biotin-avidin technique for the localisation of membrane-bound monoclonal antibodies by low power transmission electron microscopy.
      Volsen, S G
      J. Immunol. Methods, 72: 119-26 (1984)  1984

      Show Abstract
      6379052 6379052

      Data Sheet

      Title
      Sheep anti-Rabbit IgG, (H+L) Rhodamine Conjugated Affinity Purified F(ab')2 - Data Sheet

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      Alternative Format

      Catalogue Number Description  
      AQ301F Sheep Anti-Rabbit IgG Antibody, F(ab')2, FITC conjugate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies