AP510B | Sheep Anti-Rabbit IgG, Biotin-SP Conjugate Antibody

1 mg  
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      Replacement Information
      Catalogue NumberAP510B
      Trade Name
      • Chemicon
      DescriptionSheep Anti-Rabbit IgG, Biotin-SP Conjugate Antibody
      Product Information
      • Purified mouse and rabbit IgG
      PresentationPurified sheep polyclonal in PBS containing 15 mg/mL BSA and 0.1% sodium azide.
      ApplicationSheep anti-Rabbit IgG, Biotin-SP Conjugate Antibody is an antibody against Rabbit IgG for use in IHC, ELISA.
      Key Applications
      • Immunohistochemistry
      • ELISA
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution of Sheep anti-Rabbit IgG, Biotin-SP Conjugate from a representative lot was used as a secondary antibody to detect Rabbit Anti-Sox11 in mouse cerebellum and hippocampus tissues.
      Biological Information
      ImmunogenRabbit IgG
      Concentration1 mg/mL
      SpecificityThis antibody reacts with rabbit IgG and rabbit IgG (Fc) as demonstrated by ELISA. Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Purification MethodOctanoic Acid and Ammonium Sulfate cut. Depleted against Goat IgG and Rabbit IgM
      Molecular Weight~53 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.2 µg/mL of this antibody on 10 µg of purified mouse and rabbit IgG.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Secondary Antibodies