03-232 Ubiquitin activating enzyme E1 (His-tag) human recombinant

50 µg  
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      Replacement Information

      Key Spec Table

      Key ApplicationsSpeciesUni Prot Number
      Enzyme AssaysHumanP22314
      Catalogue Number03-232
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbiquitin activating enzyme E1 (His-tag) human recombinant
      OverviewHuman recombinant Ubiquitin activating enzyme E1 (His-tag)
      Background InformationUbiquitin activating enzyme E1 is responsible for the first step in the formation of the ubiquitin-protein isopeptide bond. It is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating, with ATP, the C-terminal glycine carboxyl group of ubiquitin and thenlinking this residue to the sulphydryl side chain moiety of a cysteine residue in E1, by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
      Product Information
      PresentationIn liquid containing 50 mM Tris-HCl, pH 7.5, containing 1.0 mM DTT. Frozen solution.
      ApplicationHuman recombinant Ubiquitin activating enzyme E1 (His-tag).
      Key Applications
      • Enzyme Assays
      Biological Information
      HostE. coli
      SourceFull length human ubiquitin-activating enzyme E1 containing a C-terminal His-tag
      UniProt Number
      Molecular Weight~119 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information