For fast, reproducible and gentle extraction of active proteins from yeast and plant cells, use YeastBuster™ Protein Extraction Reagent. This reagent avoids the harsh conditions of vigorous mechanical or chemical treatment that often result in degradation of target proteins. Harvest cells and resuspend in YeastBuster™ protein extraction reagent, incubate for 15 minutes and then remove cell debris by centrifugation. Your extract is now ready to use. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
Features & Benefits
Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
Higher yield of total and enzymatically active proteins
Efficient protein extraction from yeast under non-denaturing conditions from any volume of culture
Add 0.5 M THP Solution (included) and Benzonase® Nuclease for enhanced efficiency
Fully compatible with Ni-NTA His•Bind® and GST•Bind™ affinity purification method
Protein Extraction, Protein Sample Preparation, Yeast Cell Lysis, Plant Cell Lysis