ZipTip® Pipette Tips

Concentrating and purifying samples for MALDI-ToF MS

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Overview

Specifications

Ordering Information

ZipTip® Pipette Tips Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
ZTC04S008ZipTip with 0.6 µL C4 resin 8 Show Pricing & Availability
ZTC04S096ZipTip with 0.6 µL C4 resin 96 Show Pricing & Availability
ZTC04S960ZipTip with 0.6 µL C4 resin 960 Show Pricing & Availability
ZTC18M008ZipTip with 0.2 µL C18 resin 8 Show Pricing & Availability
ZTC18M096ZipTip with 0.2 µL C18 resin 96 Show Pricing & Availability
ZTC18M960ZipTip with 0.2 µL C18 resin 960 Show Pricing & Availability
ZTC18S008ZipTip with 0.6 µL C18 resin 8 Show Pricing & Availability
ZTC18S096ZipTip with 0.6 µL C18 resin 96 Show Pricing & Availability
ZTC18S960ZipTip with 0.6 µL C18 resin 960 Show Pricing & Availability
ZTSCXS008ZipTip with 0.6 µL strong cation resin 8 Show Pricing & Availability
ZTSCXS096ZipTip with 0.6 µL strong cation resin 96 Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    Purification of Radiolabeled Oligonucleotides Using ZipTipC18 Pipette Tips

    Data Sheet

    Title
    Mass Spectrometry Workflow Solutions

    Posters

    Title
    Enhanced MALDI-TOF MS Spectra of Serum Peptides Using Ultrafiltration ; ASMS 2002
    Poster: Purification of Peptides from Serum and Cell Lysates for Mass Spectrometry, ASMS, 2004
    Zip....Tips: Creative Ways to Use ZipTip Pipette Tips (ABRF 2003)
    ZipTip Chemistries Poster: Micro-Adsorptive Sample Preparation for Mass Spectrometry

    References

    Reference overviewApplicationPub Med ID
    A CROSS-SPECIES STUDY OF PI3K PROTEIN-PROTEIN INTERACTIONS REVEALS THE DIRECT INTERACTION OF P85 AND SHP2
    Susanne B. Breitkopf, Xuemei Yang, Michael J. Begley, Meghana Kulkarni, Yu-Hsin Chiu, Alexa B. Turke, Jessica Lauriol, Min Yuan, Jie Qi, Jeffrey A. Engelman, Pengyu Hong, Maria I. Kontaridis, Lewis C. Cantley, Norbert Perrimon, John M. Asara
    Sci Rep.  2016

    Show Abstract
    26839216 26839216
    FOOD PEPTIDOMICS OF IN VITRO GASTROINTESTINAL DIGESTIONS OF PARTIALLY PURIFIED BOVINE HEMOGLOBIN: LOW-RESOLUTION VERSUS HIGH-RESOLUTION LC-MS/MS ANALYSES
    Juliette Caron, Gabrielle Chataigne, Jean-Pascal Gimeno, Nathalie Duhal, Jean-Francois Goossens, Pascal Dhulster, Benoit Cudennec, Rozenn Ravallec, Christophe Flahaut
    Electrophoresis  2016

    Show Abstract
    26990205 26990205
    Comparative evaluation of peptide desalting methods for salivary proteome analysis.
    Nico Jehmlich, Claas Golatowski, Annette Murr, Gesell Salazar, Vishnu Mukund Dhople, Elke Hammer, Uwe Volker
    Clin Chim Acta  2014

    24751662 24751662
    ISOLATION AND IDENTIFICATION OF MEMBRANE VESICLE-ASSOCIATED PROTEINS IN GRAM-POSITIVE BACTERIA AND MYCOBACTERIA
    Rafael Prados-Rosales, Lisa Brown, Arturo Casadevall, Sandra Montalvo-Quiros, Jose L. Luque-Garcia
    MethodsX.  2014

    Show Abstract
    26150943 26150943
    Using theoretical protein isotopic distributions to parse small-mass-difference post-translational modifications via mass spectrometry.
    Timothy W. Rhoads, Jared R. Williams, Nathan I. Lopez, Jeffrey T. Morre, C. Samuel Bradford, Joseph S. Beckman
    J Am Soc Mass Spectrom  2013

    23247967 23247967
    DETERMINIG IN VIVO PHOSPHORYLATION SITES USING MASS SPECTROMETRY
    Susanne B. Breitkopf, John M. Asara
    Curr Protoc Mol Biol  2012

    Show Abstract
    22470061 22470061
    Rapid metabolite identification with sub parts-per-million mass accuracy from biological matrices by direct infusion nanoelectrospray ionization after clean-up on a ZipTip and LTQ/Orbitrap mass spectrometry.
    John C. L. Erve, William DeMaio, Rasmy E. Talaat
    Rapid Commun Mass Spectrom  2008

    Show Abstract
    18763271 18763271
    COMPARISON OF SDS- AND METHANOL-ASSISTED PROTEIN SOLUBILIZATION AND DIGESTION METHODS FOR ESCHERICHIA COLI MEMBRANE PROTEOME ANALYSIS BY 2-D LC-MS/MS
    Nan Zhang, Rui Chen, Nelson Young, David Wishart, Philip Winter, Joel H. Weiner, Liang Li
    Proteomics  2007

    17309111 17309111
    Analysis of modified apolipoprotein B-100 structures formed in oxidized low-density lipoprotein using LC-MS/MS.
    Takashi Obama, Rina Kato, Yutaka Masuda, Katsuhiko Takahashi, Toshihiro Aiuchi, Hiroyuki Itabe
    Proteomics  2007

    Show Abstract
    17549798 17549798
    GEL-FREE SAMPLE PREPARATION FOR THE NANOSCALE LC-MS/MS ANALYSIS AND IDENTIFICATION OF LOW-NANOGRAM PROTEIN SAMPLES
    Marco Gaspari, Vittorio Abbonante, Giovanni Cuda
    J Sep Sci.  2007

    Show Abstract
    17763504 17763504

    FAQ

    QuestionAnswer
    Can I use ZipTipC18 or ZipTipµ-C18 for amino acids?ZipTipC18 and ZipTipµ-C18exhibit comparable binding properties to C18 reversed-phase HPLC. As such, lower recoveries will be seen for hydrophilic amino acids.

    C18 is offered in two bed volumes:

    ZipTipC18 - a standard bed of 0.6 µL for sample elution in 1 to 4 µL
    ZipTipµ-C18 - a micro bed of 0.2 µL for sample elution in < 1 µL.
    How are ZipTip pipette tips packaged?ZipTip pipette tips are available in four pack sizes:
    • 8 Pack Resealable Tray
    • 24 Pack Resealable Tray
    • 96 Pack 96 place tip rack
    • 960 Pack 10 x 96 place tip rack
    How can I elute from ZipTip pipette tips directly into a nanospray needle?We suggest cutting a gel loading tip (Eppendorf 0030 001 222) approximately 2-3 mm above where the tip widens from the capillary end. ZipTip binding, washing and elution are performed utilizing the standard protocols listed in the ZipTip Reversed-Phase Operating Manual. Prior to the final dispense (elution of the peptide or protein) the cut down gel loading tip is pressed on to the end of the ZipTip using a slight twisting motion. The friction fit is tight, allowing sample to be dispensed directly into an nanospray needle.

    Refer to the ZipTip Reversed-Phase Operators Manual for a detailed diagram demonstrating the above procedure.
    How can I improve salt removal with ZipTipC18, ZipTipµ-C18 or ZipTipC4?Wash ZipTip with 3 to 5 aspirate/dispense cycles of 0.1% TFA (10 ml). If salt contamination persists, consider the following: For Peptides - Try washing peptides with 10% ACN (acetonitrile)/0.1% TFA. For proteins, the above ACN concentration can be increased to 30% Add 100 microliters of additional wash solution to a pipet and slowly push this solution through the Zip Tip. If the problem persists try blotting the Zip Tip on a paper towel to remove the residual wash soulution remainig in the Zip Tip after dispensing the final wash.
    I'm performing electrospray / nanospray mass spec on my eluted sample from ZipTip. What is the appropriate solution to elute my sample with?Peptides - 50% MeOH/0.1% TFA or 0.1% formic acid in Milli-Q water Proteins - 75% MeOH/0.1% TFA or 0.1% formic acid in Milli-Q water
    I have a polypeptide that has a molecular weight greater than 50,000 Da. Which ZipTip should I use?ZipTipC18 or ZipTipµ-C18 is most applicable for low molecular weight proteins and peptides (up to 20,000 Da) while ZipTipC4 is most suitable for low to intermediate molecular weight proteins (3,000 - 100,000 Da). In many cases, the two devices could be used interchangeably. Because higher molecular weight proteins tend to adsorb tenaciously to hydrophobic surfaces the ZipTipC4 is recommended for proteins over 100,000 Da.
    I have detergent in my sample; will I be able to enrich my peptide digest with ZipTips?Detergents will bind and concentrate with the peptides when using Reverse Phase tips such as ZipTipC18. Non-ionic and Zwitterionic detergents such as Tween-20, Chaps and triton cna be removed by ZipTipSCX at concentrations up to 0.1%. Ionic detergents such as SDS cannot be removed by ZipTips.
    I have pipetted my sample onto the ZipTip. During the elution step, an air bubble was introduced into the tip. Will this decrease the recovery of peptides?ZipTip is equipped with microchannels which travel up through the resin bed. Any air that is inadvertently introduced to the ZipTip can easily be flushed out.
    I would like to separate a peptide from a protein. Is it possible to fractionate using ZipTip?Yes, this can be accomplished by utilizing the step gradient elution protocol found in the ZipTip Reversed-Phase Operators Manual or Technical Note# TN226 entitled "Fractionation of Complex Peptide or Protein Mixtures Prior to MALDI-TOF MS Using ZipTipC18, ZipTipµ-C18 and ZipTipC4 Pipette Tips". The protocol includes multiple elution steps with increasing concentrations of acetonitrile in the elution buffer (e.g. 10%, 30%, 50%). The smaller molecules (weak binders) are eluted off the resin first and the larger molecules (strong binders) are eluted last.
    Is it possible to use plasma with Zip Tip prior to mass spec?Yes. The plasma needs to be diluted 1:4 and acidified (for example, a 1:1:2 mix of serum: 10% TFA : water).

    User Guides

    Title
    ZipTip SCX Pipette Tips