219347 | Cathepsin K Detection Kit

219347
  
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      Overview

      Replacement Information

      Key Spec Table

      Detection Methods
      Fluorescence
      Description
      Overview

      This product has been discontinued.





      Suitable for detection of cathepsin K in suspension or adherent cells. Detects cathepsin K using a cell-permeable cresyl violet-labeled cathepsin K substrate, which generates red fluorescence upon cleavage.
      Catalogue Number219347
      Brand Family Calbiochem®
      Materials Required but Not Delivered Cultured cells with medium
      Reagents to induce apoptosis or other experimental condition
      15 ml polypropylene centrifuge tube (1 per sample)
      Amber vials or polypropylene tubes for storage of 260X concentrate aliquots at -20°C
      Slides and coverslips
      Hemocytometer
      Clinical centrifuge at 200 X g
      37°C CO2 incubator
      Pipette(s) capable of dispensing at 10, 50, 200, 300 µl, and 1 ml
      ddH2O, up to 2 ml needed
      Phosphate buffered saline (PBS) pH 7.4, up to 100 ml needed
      Dimethyl sulfoxide (DMSO)
      Trypsin-EDTA
      Ice buckets or a refrigerator to maintain cells at 2°C to 8°C
      Fluorescence microscope with appropriate filters: excitation 550 nm, emission >610 nm for Cathepsin K fluorogenic substrate; excitation at 480 nm and emission at >540 nm for AO; and if Hoechst is used, a UV-filter with excitation at 365 nm, emission at 480 nm.
      References
      ReferencesBoonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473.
      Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
      Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
      Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
      Kas J. et al. 2000. Int. J. Biol. Markers 15, 84.
      Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98.
      Frosch B.A., et al. 1999. APMIS 107, 28.
      Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71.
      Bossard M.J., 1996. J. Biol. Chem. 271, 12524.
      Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135.
      Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185.
      Buhling F., et al. Adv. Exp. Med. Biol. 477, 281.
      Leung-Toung R., et al. Med. Chem. 9, 979.
      Product Information
      Detection methodFluorescence
      FormatFluorescence microscopy
      Kit containsLabeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol.
      Applications
      Biological Information
      Assay time4-6 h
      Sample TypeCells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended useThe Calbiochem® Cathepsin K Detection Kit is intended for the non-quantitative measurement of Cathepsin K activity in intact cells.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsLabeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol.
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      219347

      References

      Reference overview
      Boonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473.
      Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
      Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
      Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
      Kas J. et al. 2000. Int. J. Biol. Markers 15, 84.
      Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98.
      Frosch B.A., et al. 1999. APMIS 107, 28.
      Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71.
      Bossard M.J., 1996. J. Biol. Chem. 271, 12524.
      Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135.
      Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185.
      Buhling F., et al. Adv. Exp. Med. Biol. 477, 281.
      Leung-Toung R., et al. Med. Chem. 9, 979.

      User Protocols

      Title
      User Protocol