219347 | Cathepsin K Detection Kit

219347
  
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      Overview

      Key Spec Table

      Detection Methods
      Fluorescence
      Description
      Overview

      This product has been discontinued.





      Suitable for detection of cathepsin K in suspension or adherent cells. Detects cathepsin K using a cell-permeable cresyl violet-labeled cathepsin K substrate, which generates red fluorescence upon cleavage.
      Catalogue Number 219347
      Brand Family Calbiochem®
      References
      References Boonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473.
      Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
      Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
      Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
      Kas J. et al. 2000. Int. J. Biol. Markers 15, 84.
      Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98.
      Frosch B.A., et al. 1999. APMIS 107, 28.
      Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71.
      Bossard M.J., 1996. J. Biol. Chem. 271, 12524.
      Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135.
      Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185.
      Buhling F., et al. Adv. Exp. Med. Biol. 477, 281.
      Leung-Toung R., et al. Med. Chem. 9, 979.
      Product Information
      Detection method Fluorescence
      Format Fluorescence microscopy
      Kit contains Labeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol.
      Applications
      Biological Information
      Assay time 4-6 h
      Sample Type Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended use The Calbiochem® Cathepsin K Detection Kit is intended for the non-quantitative measurement of Cathepsin K activity in intact cells.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze No
      Packaging Information
      Transport Information
      Supplemental Information
      Kit contains Labeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol.
      Specifications

      Documentation

      MSDS

      Languages
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      Certificates of Analysis

      TitleLot Number
      219347

      References

      Reference overview
      Boonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473.
      Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
      Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
      Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
      Kas J. et al. 2000. Int. J. Biol. Markers 15, 84.
      Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98.
      Frosch B.A., et al. 1999. APMIS 107, 28.
      Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71.
      Bossard M.J., 1996. J. Biol. Chem. 271, 12524.
      Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135.
      Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185.
      Buhling F., et al. Adv. Exp. Med. Biol. 477, 281.
      Leung-Toung R., et al. Med. Chem. 9, 979.

      User Protocols

      Title
      User Protocol