400052 | Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

400052
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      Overview

      Key Spec Table

      Empirical FormulaCAS #
      C₂₀H₃₇N₃O₁₃31282-04-9

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      400052-20ML
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          20 ml
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          400052-50ML
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              50 ml
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              400052-5ML
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                  5 ml
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                  Description
                  Overview Sterile liquid formulation of Cat. No. 400050.
                  Catalogue Number 400052
                  Brand Family Calbiochem®
                  References
                  References Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Santerre, R.F., et al. 1984. Gene 30, 147.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                  Product Information
                  CAS number 31282-04-9
                  Form Liquid
                  Formulation 50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
                  Hill Formula C₂₀H₃₇N₃O₁₃
                  Chemical formula C₂₀H₃₇N₃O₁₃
                  Molar Mass 527.5
                  Structure formula Image
                  Applications
                  Biological Information
                  Biological activity Potency: ≥1000 U/mg
                  Purity ≥85% by HPLC and TLC
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  RTECS WK2130000
                  Safety Information
                  R Phrase R: 26/27/28-37/38-41

                  Very toxic by inhalation, in contact with skin and if swallowed.
                  Irritating to respiratory system and skin.
                  Risk of serious damage to eyes.
                  S Phrase S: 23-26-36/37/39-45

                  Do not breathe fumes.
                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable protective clothing, gloves and eye/face protection.
                  In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Highly Toxic & Carcinogenic / Teratogenic
                  Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                  Storage +2°C to +8°C
                  Do not freeze No
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  MSDS

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                  Certificates of Analysis

                  TitleLot Number
                  400052

                  References

                  Reference overview
                  Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Santerre, R.F., et al. 1984. Gene 30, 147.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

                  Brochure

                  Title
                  Antibiotics Profiler
                  Hygromycin B Technical Bulletin
                  Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision 04-October-2007 RFH
                  Description Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
                  Form Liquid
                  Formulation 50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
                  CAS number 31282-04-9
                  RTECS WK2130000
                  Molar mass 527.5
                  Chemical formula C₂₀H₃₇N₃O₁₃
                  Structure formula
                  Purity ≥85% by HPLC and TLC
                  Biological activity Potency: ≥1000 U/mg
                  Storage +2°C to +8°C
                  Do Not Freeze No
                  Toxicity Highly Toxic & Carcinogenic / Teratogenic
                  Merck USA index 14, 4852
                  References Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Santerre, R.F., et al. 1984. Gene 30, 147.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

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                  400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Show Pricing & Availability
                  400053 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Show Pricing & Availability

                  Categories

                  Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
                  Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents