121937 | Immobilized Anti-Mouse IgG

121937
  
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      Overview

      Description
      Overview

      This product has been discontinued.





      Designed to assist in purification or removal of mouse IgG. Contains 1-2 mg affinity-purified anti-mouse IgG/ml beaded agarose matrix. Binding capacity: approximately 1-2 mg mouse IgG/ml gel.
      Catalogue Number 121937
      Brand Family Calbiochem®
      References
      Product Information
      Form Liquid
      Formulation Supplied as a suspension in PBS.
      Preservative ≤0.1% sodium azide
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

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      Certificates of Analysis

      TitleLot Number
      121937
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision 17-July-2007 RFH
      Description Anti-mouse Ig conjugated to agrose. Designed for in purification or removal of mouse IgG.
      Form Liquid
      Formulation Supplied as a suspension in PBS.
      Preservative ≤0.1% sodium azide
      Comments Contains 1-2 mg affinity-purified anti-mouse IgG/ml beaded agarose matrix. Binding capacity: ~1-2 mg mouse IgG/ml gel.

      Directions for Use in Immunoaffinity Chromatography

      1. Without disturbing the gel bed, carefully apply antiserum, ascitic fluid, or tissue culture supernatant to a column of immobilized anti-mouse IgG. Let the fluid slowly penetrate the column.
      2. Wash the column with 10 mM Na2HPO4, pH 7.2-7.4, containing 150 mM NaCl, pH 7.4. Continue washing the column until a baseline absorbance at 280 nm is reached.
      3. Elute the column with 100 mM acetic acid, containing 300 mM NaCl, pH 2.4. The eluant should be neutralized immediately with 0.1 N NaOH. Continue to elute until the absorbance returns to baseline levels.
      4. Regenerate the column by applying wash buffer at 5X the column volume.
      5. To exchange buffers, use a desalting gel (e.g. Sephadex G-25).

      Conditions should be optimized for idividual experiments.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling