440202 | LY 294002 - CAS 154447-36-6 - Calbiochem

440202
Price could not be retrieved
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability Limited Availability
Discontinued
There is a newer version of this product
Limited Quantities Available
Availability to be confirmed
      Will advise
      View Pricing & Availability
         CompareClick To Print This Page

      Overview

      Key Spec Table

      Empirical FormulaCAS #
      C₁₉H₁₇NO₃154447-36-6

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      440202-10MG
      Retrieving availability...
      Limited Availability Limited Availability
      Discontinued
      There is a newer version of this product
      Limited Quantities Available
      Availability to be confirmed
          Will advise

          10 mg
          Retrieving price...
          Price could not be retrieved
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          440202-25MG
          Retrieving availability...
          Limited Availability Limited Availability
          Discontinued
          There is a newer version of this product
          Limited Quantities Available
          Availability to be confirmed
              Will advise

              25 mg
              Retrieving price...
              Price could not be retrieved
              Upon Order Completion More Information
              You Saved ()
               
              Request Pricing
              440202-5MG
              Retrieving availability...
              Limited Availability Limited Availability
              Discontinued
              There is a newer version of this product
              Limited Quantities Available
              Availability to be confirmed
                  Will advise

                  5 mg
                  Retrieving price...
                  Price could not be retrieved
                  Upon Order Completion More Information
                  You Saved ()
                   
                  Request Pricing
                  Description
                  Overview A cell-permeable, potent, reversible and specific phosphatidylinositol 3-kinase inhibitor (IC50 = 1.4 µM) that acts on the ATP-binding site of the enzyme. Also inhibits nonhomologous DNA end-joining (NHEJ) activity of the 460 kDa phosphatidylinositol 3-like kinase DNA-PKcs, which is the catalytic subunit of DNA-activated protein kinase. Does not affect the activities of EGF receptor kinase, MAP kinase, PKC, Pl 4-kinase, S6 kinase, and c-Src even at 50 µM. Promotes the nuclear accumulation of active GSK-3b. Reported to suppress the proliferation of murine embryonic stem cells. Blocks the proliferation of cultured rabbit aortic smooth muscle cells without inducing apoptosis. Shown to bind to the same site as I-BET (Cat. No. 40101050 ~ 10 µM). A 10 mM (1 mg/325 µl) solution of LY 294002 (Cat. No. 440204) in DMSO is also available.
                  Catalogue Number 440202
                  Brand Family Calbiochem®
                  Synonyms 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, BRD2 Inhibitor IV, BRD3 Inhibitor III, BRD4 Inhibitor IV
                  References
                  References Dittman, A., et al. 2013. ACS Chem. Biol. 9, 495.
                  Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
                  Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
                  Baumann, P., and West, S.C. 1998. Proc. Natl. Acad. Sci. USA 95, 14066.
                  Cardone, M.H., et al. 1998. Science 282, 1318.
                  Vlahos, C.J., et al. 1995. J. Immunol. 154, 2413.
                  Yano, H., et al. 1995. Biochem. J. 312, 145.
                  Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.



                  Selected Citations
                  Lee, J., et al. 2009. Cell Stem Cell 5, 76.


                  Product Information
                  CAS number 154447-36-6
                  ATP Competitive Y
                  Form Off-white solid
                  Hill Formula C₁₉H₁₇NO₃
                  Chemical formula C₁₉H₁₇NO₃
                  Molar Mass 307.4
                  Reversible Y
                  Structure formula Image
                  Applications
                  Biological Information
                  Primary Target Phosphatidylinositol 3-kinase
                  Primary Target IC<sub>50</sub> 1.4 µM against phosphatidylinositol 3-kinase
                  Purity ≥98% by HPLC
                  Physicochemical Information
                  Cell permeable Y
                  Solubility DMSO or EtOH
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Standard Handling
                  Storage -20°C
                  Protect from Light Protect from light
                  Do not freeze No
                  Special Instructions Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  MSDS

                  Languages
                  Danmark Deutschland Ellas
                  English España France
                  Italia Korea Magyarország
                  Nederland  Norge Polska
                  Portugal Suomi Sverige
                  България

                  Certificates of Analysis

                  TitleLot Number
                  440202

                  References

                  Reference overview
                  Dittman, A., et al. 2013. ACS Chem. Biol. 9, 495.
                  Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
                  Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
                  Baumann, P., and West, S.C. 1998. Proc. Natl. Acad. Sci. USA 95, 14066.
                  Cardone, M.H., et al. 1998. Science 282, 1318.
                  Vlahos, C.J., et al. 1995. J. Immunol. 154, 2413.
                  Yano, H., et al. 1995. Biochem. J. 312, 145.
                  Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.



                  Selected Citations
                  Lee, J., et al. 2009. Cell Stem Cell 5, 76.


                  Citations

                  Title
                • Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
                • Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
                • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
                • Satoshi Fukumoto, et al. (2006) Laminin 5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. Journal of Biological Chemistry 281, 5008-5016.
                • Ana Olivera, et al. (2006) IgE-dependent activation of sphingosine kinase 1 and 2 and secretion of sphingosine-1-phosphate requires Fyn kinase and contributes to mast cell responses. Journal of Biological Chemistry 281, 2515-2525.
                • Qian Yang, et al. (2006) TSC1/TSC2 and Rheb have different effects on TORC1 and TORC2 activity. Proceedings of the National Academy of Sciences (USA) 103, 6811-6816.
                • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
                • Huanyu Dou, et al. (2005) Neuroprotective mechanisms of lithium in murine human immunodeficiency virus-1 encephalitis. Journal of Neuroscience 25, 8375-8385.
                • Julie Lotharius, et al. (2005) Progressive degeneration of human mesencephalic neuron-derived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed lineage kinase pathway. Journal of Neuroscience 25, 6329-6342.
                • Meenalakshmi M. Mariappan, M.M., et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
                • Michael Tscharntke, et al. (2005) Ras-induced spreading and wound closure in human epidermal keratinocytes. The FASEB Journal in press,.
                • Junji Tsurutani, et al. (2005) Inhibition of the phosphatidylinositol 3-kinase/Akt/Mammalian target of rapamycin pathway but no the MEK/ERK Pathway attentuates laminin-meidated small cell lunch cancer cellular survival and resistance to Imatinib mesylate or chemolyte.. Cancer Research 65, 8423-8432.
                • Xinbo Liao, et al. (2003) Androgen stimulates matrix metalloproteinase-2 expression in human prostate cancer. Endocrinology 144, 1656-1663.
                • Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision 28-February-2012 RFH
                  Synonyms 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, BRD2 Inhibitor IV, BRD3 Inhibitor III, BRD4 Inhibitor IV
                  Description A cell-permeable, potent, reversible, and specific phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor that acts on the ATP-binding site of the enzyme (IC50 = 1.4 µM). Also inhibits nonhomologous DNA end-joining (NHED) in the 460 kDa phosphatidylinositol 3-like kinase DNA-PKcs, which is the catalytic subunit of DNA-activated protein kinase. Does not affect the activity of EGF receptor kinase, MAP kinase, PKC, PI 4-kinase, S6 kinase, and c-src at 50 µM. Promotes the nuclear accumulation of active GSK-3b. Reported to suppress the proliferation of murine embryonic stem cells. Blocks the proliferation of cultured rabbit aortic smooth muscle cells without inducing apoptosis. Reported to inhibit histamine secretion in RBL-2H3 cells (IC50 = 5 µM).
                  Form Off-white solid
                  CAS number 154447-36-6
                  Molar mass 307.4
                  Chemical formula C₁₉H₁₇NO₃
                  Structure formula
                  Purity ≥98% by HPLC
                  Solubility DMSO (20 mg/ml) or Ethanol. Slight warming may be required to achieve complete solubilization.
                  Storage -20°C
                  Protect from light
                  No
                  Special Instructions Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
                  Toxicity Standard Handling
                  References Dittman, A., et al. 2013. ACS Chem. Biol. 9, 495.
                  Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
                  Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
                  Baumann, P., and West, S.C. 1998. Proc. Natl. Acad. Sci. USA 95, 14066.
                  Cardone, M.H., et al. 1998. Science 282, 1318.
                  Vlahos, C.J., et al. 1995. J. Immunol. 154, 2413.
                  Yano, H., et al. 1995. Biochem. J. 312, 145.
                  Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.



                  Selected Citations
                  Lee, J., et al. 2009. Cell Stem Cell 5, 76.


                  Citation
                • Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
                • Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
                • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
                • Satoshi Fukumoto, et al. (2006) Laminin 5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. Journal of Biological Chemistry 281, 5008-5016.
                • Ana Olivera, et al. (2006) IgE-dependent activation of sphingosine kinase 1 and 2 and secretion of sphingosine-1-phosphate requires Fyn kinase and contributes to mast cell responses. Journal of Biological Chemistry 281, 2515-2525.
                • Qian Yang, et al. (2006) TSC1/TSC2 and Rheb have different effects on TORC1 and TORC2 activity. Proceedings of the National Academy of Sciences (USA) 103, 6811-6816.
                • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
                • Huanyu Dou, et al. (2005) Neuroprotective mechanisms of lithium in murine human immunodeficiency virus-1 encephalitis. Journal of Neuroscience 25, 8375-8385.
                • Julie Lotharius, et al. (2005) Progressive degeneration of human mesencephalic neuron-derived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed lineage kinase pathway. Journal of Neuroscience 25, 6329-6342.
                • Meenalakshmi M. Mariappan, M.M., et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
                • Michael Tscharntke, et al. (2005) Ras-induced spreading and wound closure in human epidermal keratinocytes. The FASEB Journal in press,.
                • Junji Tsurutani, et al. (2005) Inhibition of the phosphatidylinositol 3-kinase/Akt/Mammalian target of rapamycin pathway but no the MEK/ERK Pathway attentuates laminin-meidated small cell lunch cancer cellular survival and resistance to Imatinib mesylate or chemolyte.. Cancer Research 65, 8423-8432.
                • Xinbo Liao, et al. (2003) Androgen stimulates matrix metalloproteinase-2 expression in human prostate cancer. Endocrinology 144, 1656-1663.