115943 | Methylene blue (C.I.52015)

115943
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      Overview

      Replacement Information

      Key Spec Table

      EC Number Hill Formula Chemical Formula Molar Mass
      200-515-2C₁₆H₁₈ClN₃S * x H₂OC₁₆H₁₈ClN₃S * x H₂O (x=2-3)319.86 g/mol (anhydrous)

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      1159430025
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          Glass bottle 25 g
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          1159430100
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              Glass bottle 100 g
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              Description
              Catalogue Number 115943
              Synonyms Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
              Description METHYLENE BLUE (C.I. 52015) FOR MICROSCO
              Overview Methylene blue (C.I. 52015) is a Certistain® dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration.
              References
              Product Information
              EC number 200-515-2
              Hill Formula C₁₆H₁₈ClN₃S * x H₂O
              Chemical formula C₁₆H₁₈ClN₃S * x H₂O (x=2-3)
              Molar Mass 319.86 g/mol (anhydrous)
              HS Code 3204 13 00
              Structure formula Image
              Applications
              Biological Information
              Physicochemical Information
              Melting Point 180 °C (decomposition)
              pH value 3 (10 g/l, H₂O, 20 °C)
              Bulk density 400 - 600 kg/m3
              Solubility 50 g/l (20 °C)
              Dimensions
              Materials Information
              Toxicological Information
              LD 50 oral LD50 Rat 1180 mg/kg
              Safety Information according to GHS
              Hazard Pictogram(s)
              Hazard Statement(s) H302: Harmful if swallowed.
              Signal Word Warning
              Storage class 10 - 13 Other liquids and solids
              WGK WGK 2 water endangering
              Disposal 3
              Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
              Safety Information
              Hazard Symbols Harmful
              Categories of danger harmful
              R Phrase R 22
              Harmful if swallowed.
              Product Usage Statements
              Storage and Shipping Information
              Storage Store at +5°C to +30°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Specifications
              Dye content (spectrophotometrically)≥ 82 %
              Identity
              - Chloride

              passes test
              - UV/VIS-Spectrumpasses test
              Absorption maximum λ max (ethanol 50 %)660 - 665 nm
              Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2250 - 2750
              TLC-Testpasses test
              Loss on drying (110 °C)10 - 15 %
              Suitability for microscopypasses test

              Documentation

              MSDS

              Title

              Material Safety Data Sheet (MSDS)  

              Certificates of Analysis

              Product Number Packaging Specification Lot Number
              1159430025 Glass bottle PDF Specification Document
              1159430100 Glass bottle PDF Specification Document

              Brochure

              Title
              Alles für die Histologie. Schnell und zuverlässig.
              Fast and reliable. All you need for histology.
              Products for microscopy - All you need for successful working

              Reach Use

              Title
              Reach Use

              Instructions for Use

              Title
              115943 Methylene blue (C.I. 52015) for microscopy Certistain®

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              Categories

              IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
              Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Redox Indicators