14-251 | p38α/SAPK2a Protein, active, 10 µg

14-251
10 µg  Also available in 250μg size (2x125μg) - Call for pricing and availability. Please reference catalog number 14-251M when ordering the 250μg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-251
      Brand Family Upstate
      Trade Name
      • Upstate
      Descriptionp38α/SAPK2a Protein, active, 10 µg
      OverviewN-terminal GST tagged, recombinant, full length p38α/SAPK2a
      References
      Product Information
      Applications
      ApplicationActive, N-terminal GST tagged, recombinant, full length p38α/SAPK2a, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
      Gene Symbol
      • MAPK14
      • PRKM14
      • RK
      • SAPK2A
      • CSBP1
      • p38
      • CSPB1
      • CSBP
      • PRKM15
      • Mxi2
      • p38ALPHA
      • MXI2
      • EXIP
      • CSBP2
      Protein Targetp38α/SAPK2a
      Purification MethodGlutathione-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
      COFACTOR: Magnesium.
      SIZE: 360 amino acids; 41293 Da
      SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
      TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.7kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of MBP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size (2x125μg) - Call for pricing and availability. Please reference catalog number 14-251M when ordering the 250μg size.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      p38 alpha/SAPK2a , active - D8NN029U-B D8NN029U-B
      p38 alpha/SAPK2a , active - D8NN029U-C D8NN029U-C
      p38 alpha/SAPK2a, active - 1599366-H 1599366-H
      p38 alpha/SAPK2a, active - 2021335-A 2021335-A
      p38 alpha/SAPK2a, active - D8NN029U-D D8NN029U-D

      References

      Reference overviewPub Med ID
      Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells.
      Doza, Y N, et al.
      FEBS Lett., 364: 223-8 (1995)  1995

      Show Abstract
      7750576 7750576
      SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1.
      Cuenda, A, et al.
      FEBS Lett., 364: 229-33 (1995)  1995

      Show Abstract
      7750577 7750577
      A single autophosphorylation site confers oncogenicity to the Neu/ErbB-2 receptor and enables coupling to the MAP kinase pathway.
      Ben-Levy, R, et al.
      EMBO J., 13: 3302-11 (1994)  1994

      7913890 7913890

      Technical Info

      Title
      Millipore White Paper: Battle at the Gates: Combating Mutant Kinases

      FAQ

      QuestionAnswer
      Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-251M p38α/SAPK2a Protein, active, 250 µg Show Pricing & Availability

      Accessories

      Catalogue Number Description  
      20-101 Protein Dilution Buffer A Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases