14-253 | p38β2/SAPK2b2 Protein, active, 10 µg

14-253
10 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-253
      Brand Family Upstate
      Trade Name
      • Upstate
      Descriptionp38β2/SAPK2b2 Protein, active, 10 µg
      OverviewN-terminal GST fusion protein corresponding to full length human p38β2/SAPK2b2 activated with MKK6.
      References
      Product Information
      Applications
      ApplicationActive, N-terminal GST fusion protein corresponding to full length human p38β2/SAPK2b2 activated with MKK6. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourcefull length human p38β2/SAPK2b2, expressed in E. coli.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase.
      Gene Symbol
      • MAPK11
      • p38-2
      • SAPK2B
      • SAPK2
      • PRKM11
      • P38BETA2
      • P38B
      • p38b
      • p38Beta
      • p38beta2
      Protein Targetp38β2/SAPK2b2
      Purification MethodAffinity chromatography on glutathione-agarose beads
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15759 # Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
      COFACTOR: Magnesium (By similarity).
      SIZE: 364 amino acids; 41357 Da
      TISSUE SPECIFICITY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
      DOMAIN:SwissProt: Q15759 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme (Probable).
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight71kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of myelin basic protein
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      SAPK2b, active - D7DN025U-B D7DN025U-B
      SAPK2b, active - D7DN025U-C D7DN025U-C
      SAPK2b, active - D7DN025U-D D7DN025U-D
      SAPK2b, active - D8JN048U-C D8JN048U-C

      References

      Reference overviewPub Med ID
      Activation of stress-activated protein kinase-3 (SAPK3) by cytokines and cellular stresses is mediated via SAPKK3 (MKK6); comparison of the specificities of SAPK3 and SAPK2 (RK/p38).
      Cuenda, A, et al.
      EMBO J., 16: 295-305 (1997)  1997

      Show Abstract
      9029150 9029150
      SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1.
      Cuenda, A, et al.
      FEBS Lett., 364: 229-33 (1995)  1995

      Show Abstract
      7750577 7750577

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-253M p38β2/SAPK2b2 Protein, active, 250 µg Show Pricing & Availability

      Accessories

      Catalogue Number Description  
      20-101 Protein Dilution Buffer A Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases