69015 | pET-32a(+) DNA

69015
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      Overview

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      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      69015-3
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      Discontinued
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          10 μg
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          Description
          Overview The pET-32a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB122VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs. Commercial entities need to obtain a license from Brookhaven National Labs prior to use of this Product. Commercial use of this Product also requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Catalogue Number 69015
          Brand Family Novagen®
          References
          References 1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.
          Product Information
          Vector family pET
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

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          Certificates of Analysis

          TitleLot Number
          69015

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

          Citations

          Title
        • Robertson, P.D., et al. 2008. Journal of Biological Chemistry 283, 3338.
        • Cai, M., et al. 2007. Journal of Biological Chemistry 282, 14525.
        • Dai, M., et al. 2007. Plant Physiology 144, 121.
        • Adam Joelsson, A., et al. 2007. Applied and Enviornmental Microbiology 73, 3742.
        • Wong, H.L., et al. 2007. Plant Cell 19, 4022.
        • Gyan, S., et al. 2006. Journal of Bacteriology 188, 7062.
        • Masin, M., et al. 2006. Journal of Biological Chemistry 281, 4100.
        • Oertelt, S., et al. 2006. Journal of Immunology 177, 1655.
        • Smith R.M., and Williams S.B., 2006. Proceedings of the National Academy of Sciences (USA) 103, 8564.
        • Suzuki, H., et al. 2006. Journal of Biological Chemistry 281, 30251.
        • Vercauteren, K., et al. 2006. Molecular and Cellular Biology 26, 7409.
        • Anjard, C., and Loomis, W.F., 2005. Procedings of the National Academy of Science 102, 7607.
        • Bracken, C.P., et al. 2005. Journal of Biological Chemistry 280, 14240.
        • Don J. Davidson, D.J., et al. 2005. Cancer Research 65, 4663.
        • Dufour, E.K., et al. 2005. Protein Science 14, 303.
        • Hoffmeister, M., et al. 2005. Journal of Biological Chemistry 280, 4329.
        • Hymowitz, S.G., et al. 2005. Journal of Biological Chemistry 280, 7218.
        • Kani, K., et al. 2005. Journal of Biological Chemistry 280, 8238.
        • Katou, S., et al. 2005. Journal of Biological Chemistry 280, 39569.
        • Lara, M.V., et al. 2005. Plant and Cell Physiology 46, 997.
        • Lo, K.W., et al. 2005. Journal of Biological Chemistry 280, 8172.
        • Ran X., and Song J., 2005. Journal of Biological Chemistry 280, 19205.
        • Schilling, O., et al. 2005. Journal of Biological Chemistry 280, 17857.
        • Takahashi-Terada, A., et al. 2005. Journal of Biological Chemistry 280, 11798.
        • Yoshida S., and Parniske M., 2005. Journal of Biological Chemistry 280, 9203.
        • Holland M.C.H., and Lambris J.D., 2004. Journal of Immunology 172, 349.
        • Ichinoe, A., et al. 2004. i>Nucleic Acids Research 32, 477.
        • Mancini, E.J., et al. 2004.Acta Crystallographica D60, 588.
        • Raschle,M., et al. 2004. Journal of Biological Chemistry 279, 51973.
        • Trabbic-Carlson, K., et al. 2004. Protein Engineering Design and Selection 17, 57.
        • Marina V. Backer, M.V., et al. 2003. Protein Expression and Purification 26, 455.
        • Perrin, M.H., et al. 2003. Journal of Biological Chemistry 278, 15595.
        • Rosenblum, M.G., et al. 2003. Cancer Research 63, 3995.
        • Lobel, L., et al. 2002. Protein Expression and Purification 25, 124.
        • Koffa, M.D., et al. 2001. European Molecular Biology Organization Journal 20, 5769.
        • Lauber, T., et al. 2001. Protein Expression and Purification 22, 108.
        • Lobel, L.I., et al. 2001. Endocrine 14, 205.
        • Huang, J.D., et al. 1999. Nature 397, 267.
        • Becker, S., et al. 1998. nature 394, 145.
        • Xu, G.L., and Bestor T.H., 1997. Nature Genetics 17, 376.
        • User Protocols

          Title
          TB053 Academic and Non-profit Laboratory Assurance Letter
          TB055 pET System Manual

          Vector Map

          Title
          TB122VM pET-32a-c(+) Vector Map

          Vector Sequence

          Title
          pET-32a(+) Sequence

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors