69090 | pIE1-3 DNA

69090
  
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      Overview

      Description
      Overview The pIE1-3 vector is designed for constitutive expression of recombinant proteins from the baculovirus ie1 promoter in stably-transformed insect cells (1). The plasmid contains all promoter sequences known to be important for ie1-mediated gene expression in uninfected insect cells as well as the hr5 enhancer element. pIE1-3 lacks an ie1-driven translation initiation site; therefore, the ATG start codon must be supplied by the insert. To obtain stably-transformed cells, a pIE1-3 recombinant is cotransfected with pIE1-neo, which contains the Neo selectable marker under ie1 control.
      Catalogue Number 69090
      Brand Family Novagen®
      References
      References 1. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Prot. Exp. Pur. 8, 191–203.
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      Ship Code Blue Ice or Dry Ice
      Toxicity Standard Handling
      Storage FREEZER (-20°C)
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze No
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      TitleLot Number
      69090

      References

      Reference overview
      1. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Prot. Exp. Pur. 8, 191–203.

      Citations

      Title
    • Katrin Schnizler, et al. (2005) A novel chloride channel in Drosophila melanogaster is inhibited by protons. Journal of Biological Chemistry 28o, 16254-16262.