71177 | Rosetta-gami™ B Competent Cell Set

71177
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      Overview

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      71177-3
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          Description
          Overview Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

          pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
          Genotypes:
          Rosetta-gami B
          F ompT hsdSB (rB mB) gal dcm lacY1 ahpC gor522::Tn10 trxB pRARE (CamR, KanR, TetR)

          Rosetta-gami B(DE3)
          F ompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)

          Rosetta-gami B(DE3)pLysS
          F ompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (CamR, KanR, TetR)
          Catalogue Number 71177
          Brand Family Novagen®
          References
          Product Information
          Components
          2 × 0.2 mlRosetta-gami B Competent Cells
          2 × 0.2 mlRosetta-gami B(DE3) Competent Cells
          2 × 0.2 mlRosetta-gami B(DE3)pLysS Competent Cells
          4 × 2 mlSOC Medium
          2 ngTest Plasmid
          Applications
          Biological Information
          Physicochemical Information
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          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression