344291 | Freund’s Incomplete Adjuvant, Modified

344291
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      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      344291-10ML
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          10 ml
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          Descripción
          Visión de conjunto Contains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml.
          Número de catálogo 344291
          Marca de familia Calbiochem®
          Referencias bibliográficas
          Referencias bibliográficas Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.
          Información del producto
          Formulario Yellow oily liquid
          Aplicaciones
          Información biológica
          Información fisicoquímica
          Dimensiones
          Información de materiales
          Información toxicológica
          Información de seguridad según el GHS
          Información de seguridad
          Declaraciones de uso del producto
          Información de almacenamiento y transporte
          Código de envío Ambient Temperature Only
          Toxicidad Standard Handling
          Almacenamiento +15°C to +30°C
          No congelar No
          Información sobre embalaje
          Información de transporte
          Información complementaria
          Especificaciones

          Documentation

          Ficha datos de seguridad (MSDS)

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          CargoNúmero de lote
          344291

          Referencias bibliográficas

          Visión general referencias
          Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revisión 26-June-2008 RFH
          Descripción The word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
          Formulario Yellow oily liquid
          Solubilidad Composition
          10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:
          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier

          Sample Preparation
          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Comentarios 10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier.

          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Almacenamiento +15°C to +30°C
          Do Not Freeze No
          Toxicidad Standard Handling
          Referencias bibliográficas Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.