69725 | λDE3 Lysogenization Kit plus pLysS & pLysE

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          Description
          Overview The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

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          Product Information
          Components
          200 µlλDE3 Phage Lysate
          200 µlHelper Phage Lysate
          200 µlSelection Phage Lysate
          50 µlT7 Tester Phage Lysate
          200 µlHMS174(DE3) glycerol stock
          1 µgpLysS DNA
          1 µgpLysE DNA
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
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          69725

          Citas

          Título
        • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
        • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
        • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
        • Protocolos de usuario

          Cargo
          TB053 Academic and Non-profit Laboratory Assurance Letter

          Mapa vectorial

          Cargo
          TB107 pLysS and pLysE Plasmid Map

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           Número de referencia Descripción  
          69734 λDE3 Lysogenization Kit Precios y disponibilidad

          Categorías

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors