428900 | LCAT Activity Assay Kit, Fluorometric

428900
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      Overview

      Replacement Information

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      Métodos de detección
      Fluorometric

      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      428900-1KIT
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          Descripción
          Visión de conjunto Assay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol.
          Número de catálogo 428900
          Marca de familia Calbiochem®
          Sinónimos Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric
          Datos de aplicación
          The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.

          Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample.
          Referencias bibliográficas
          Información del producto
          Método de detección Fluorometric
          Formulario 100 Tests
          El kit contiene LCAT Substrate, READ Reagent, and a user protocol.
          Aplicaciones
          Información biológica
          Tiempo de ensayo 4.5 - 8.5 h
          Tipo de muestra Plasma
          Especie human
          Información fisicoquímica
          Dimensiones
          Información de materiales
          Información toxicológica
          Información de seguridad según el GHS
          Información de seguridad
          Frase R R: 36/38

          Frase S S: 26

          Declaraciones de uso del producto
          Indicaciones de uso The Calbiochem® LCAT Activity Assay Kit, Fluorometric is intended for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in normal human plasma. This kit allows the quantitation of fold of increase or decrease of enzymatic activity among samples, this kit also allows the quantitation of the % change of 470/390 ratio with time, which can be used to define LCAT enzymatic activity. This kit does not allow the measurement of substrate concentration or the amount of hydrolyzed substrate.
          Información de almacenamiento y transporte
          Código de envío Blue Ice Only
          Toxicidad Irritant
          Almacenamiento Multiple storage requirements
          Condiciones de almacenamiento Upon arrival store the LCAT Substrate at -20°C and the READ Reagent at 4°C.
          Proteger de la luz Protect from light
          No congelar No
          Información sobre embalaje
          Información de transporte
          Información complementaria
          El kit contiene LCAT Substrate, READ Reagent, and a user protocol.
          Especificaciones

          Documentation

          Ficha datos de seguridad (MSDS)

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          Certificados de análisis

          CargoNúmero de lote
          428900
          Protocolo de usuario

          Revisión 13-July-2012 JSW
          Sinónimos Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric
          Formulario 100 Tests
          Método de detección Fluorometric
          Especie human
          Almacenamiento Upon arrival store the LCAT Substrate at -20°C and the READ Reagent at 4°C.
          Indicaciones de uso The Calbiochem® LCAT Activity Assay Kit, Fluorometric is intended for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in normal human plasma. This kit allows the quantitation of fold of increase or decrease of enzymatic activity among samples, this kit also allows the quantitation of the % change of 470/390 ratio with time, which can be used to define LCAT enzymatic activity. This kit does not allow the measurement of substrate concentration or the amount of hydrolyzed substrate.
          Principios del ensayo LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol. Using the LCAT Activity Assay, Fluorometric, human plasma samples are incubated with LCAT Substrate, a fluorescent substrate that emits fluorescence at 470 nm. When the substrate is hydrolyzed by LCAT, a monomer is released that emits fluorescence at 390 nm. The LCAT activity is assessed as a change in 470/390 emission intensity.










          Figure 1: Assay Principle

          The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.

          Materiales suministrados There are sufficient reagents for 100 tests.

          •LCAT Substrate (Kit Component No. KP23001): 1 vial, 100 µl
          •READ Reagent (Kit Component No. KP23002): 1 vial, 350 µl
          Materiales requeridos, pero no suministrados •LCAT Assay Buffer (150 mM NaCl, 10 mM Tris-HCl, 4 mM 2-mercaptoethanol, 1 mM EDTA, 7.4)
          •READ Reagent Reconstitution Buffer (150 mM NaCl, 10 mM Tris-HCl, 1 mM EDTA, pH 7.4)
          Preparación del reactivo •READ Reagent: reconstitute the vial of READ Reagent with 29.7 ml READ Reagent Reconstitution Buffer.
          Protocolo detallado 1. Mix 1 µl LCAT Substrate 200 µl LCAT Assay Buffer with sample containing LCAT (e.g., 3-5 µl normal human plasma) and incubate for 4-8 h at 37°C.
          2. Add 100 µl of the mixture in step 1 to 300 µl READ Reagent and vortex. Read the fluorescence at an excitation wavelength of 340 nm and an emission wavelength of 390 nm and 470 nm. DO NOT incubate the samples in the presence of READ Reagent, as this will inactivate the LCAT in the sample.

          NOTE: The emission intensities at 390 nm and 470 nm represent the emission of the substrate hydrolyzed (390 nm) and not hydrolyzed (470 nm). In the presence of LCAT, a ratio of the two emission intensities (470/390) will indicate an increase in concentration of the monomer, which emits at 390 nm, and the simultaneous decrease in concentration of the non-hydrolyzed LCAT Substrate, which emits at 470 nm.
          Datos de ejemplo

          Figure 2: Example Data

          Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample.

          Marcas registradas Calbiochem® is a registered trademark of Merck KGaA, Darmstadt.
          Interactive Pathways™ is a trademark of Merck KGaA, Darmstadt.