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PCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.
The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR enzyme for the amplification of long or GC-rich DNA templates, including mouse tail-tips, plant tissues and synthetic biology applications. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.
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*Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.
|Features and benefits||
|Safety Information according to GHS|
Irritating to eyes, respiratory system and skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
|Product Usage Statements|
|Storage and Shipping Information|
Shipped with Blue Ice or with Dry Ice
|Do not freeze||
Ok to freeze
|Titre||Numéro de lot|
|PCR Protocols and Guides - Simplify your gene discovery (MM)|
|TB507 KOD Xtreme™ Hot Start DNA Polymerase|