428900 | LCAT Activity Assay Kit, Fluorometric

428900
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      Overview

      Tableau de caractéristiques principal

      Méthodes de détection
      Fluorometric

      Prix & Disponibilité

      RéférenceDisponibilité Conditionnement Qté Prix Quantité
      428900-1KIT
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      Disponibilité limitée Disponibilité limitée
      Interrompu(e)
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          1 kit
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          Description
          Aperçu Assay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol.
          Référence 428900
          Famille de marques Calbiochem®
          Synonymes Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric
          Données d'applications
          The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.

          Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample.
          Références bibliographiques
          Informations produit
          Méthode de détection Fluorometric
          Forme 100 Tests
          Contenu du kit LCAT Substrate, READ Reagent, and a user protocol.
          Applications
          Informations biologiques
          Temps de l'essai 4.5 - 8.5 h
          Type d'échantillon Plasma
          Espèce human
          Informations physico-chimiques
          Dimensions
          Informations sur les matériaux
          Informations toxicologiques
          Informations de sécurité selon le SGH
          Informations sur la sécurité
          Phrase R (de risques) R: 36/38

          Phrase S (de sécurité) S: 26

          Notifications sur l'utilisation du produit
          Utilisation prévue The Calbiochem® LCAT Activity Assay Kit, Fluorometric is intended for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in normal human plasma. This kit allows the quantitation of fold of increase or decrease of enzymatic activity among samples, this kit also allows the quantitation of the % change of 470/390 ratio with time, which can be used to define LCAT enzymatic activity. This kit does not allow the measurement of substrate concentration or the amount of hydrolyzed substrate.
          Informations relatives au stockage et à l'expédition
          Code Expé Blue Ice Only
          Toxicité Irritant
          Stockage Multiple storage requirements
          Conditions de stockage Upon arrival store the LCAT Substrate at -20°C and the READ Reagent at 4°C.
          Protéger de la lumière Protect from light
          Ne pas congeler No
          Informations sur l'emballage
          Informations sur le transport
          Information complémentaire
          Contenu du kit LCAT Substrate, READ Reagent, and a user protocol.
          Caractéristiques

          Documentation

          FDS

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          Certificats d'analyse

          TitreNuméro de lot
          428900
          Protocole Utilisateur

          Révision 13-July-2012 JSW
          Synonymes Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric
          Forme 100 Tests
          Méthode de détection Fluorometric
          Espèce human
          Stockage Upon arrival store the LCAT Substrate at -20°C and the READ Reagent at 4°C.
          Utilisation prévue The Calbiochem® LCAT Activity Assay Kit, Fluorometric is intended for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in normal human plasma. This kit allows the quantitation of fold of increase or decrease of enzymatic activity among samples, this kit also allows the quantitation of the % change of 470/390 ratio with time, which can be used to define LCAT enzymatic activity. This kit does not allow the measurement of substrate concentration or the amount of hydrolyzed substrate.
          Principes de l'essai LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol. Using the LCAT Activity Assay, Fluorometric, human plasma samples are incubated with LCAT Substrate, a fluorescent substrate that emits fluorescence at 470 nm. When the substrate is hydrolyzed by LCAT, a monomer is released that emits fluorescence at 390 nm. The LCAT activity is assessed as a change in 470/390 emission intensity.










          Figure 1: Assay Principle

          The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.

          Matériel fourni There are sufficient reagents for 100 tests.

          •LCAT Substrate (Kit Component No. KP23001): 1 vial, 100 µl
          •READ Reagent (Kit Component No. KP23002): 1 vial, 350 µl
          Matériel requis, mais non fourni •LCAT Assay Buffer (150 mM NaCl, 10 mM Tris-HCl, 4 mM 2-mercaptoethanol, 1 mM EDTA, 7.4)
          •READ Reagent Reconstitution Buffer (150 mM NaCl, 10 mM Tris-HCl, 1 mM EDTA, pH 7.4)
          Préparation des réactifs •READ Reagent: reconstitute the vial of READ Reagent with 29.7 ml READ Reagent Reconstitution Buffer.
          Protocole détaillé 1. Mix 1 µl LCAT Substrate 200 µl LCAT Assay Buffer with sample containing LCAT (e.g., 3-5 µl normal human plasma) and incubate for 4-8 h at 37°C.
          2. Add 100 µl of the mixture in step 1 to 300 µl READ Reagent and vortex. Read the fluorescence at an excitation wavelength of 340 nm and an emission wavelength of 390 nm and 470 nm. DO NOT incubate the samples in the presence of READ Reagent, as this will inactivate the LCAT in the sample.

          NOTE: The emission intensities at 390 nm and 470 nm represent the emission of the substrate hydrolyzed (390 nm) and not hydrolyzed (470 nm). In the presence of LCAT, a ratio of the two emission intensities (470/390) will indicate an increase in concentration of the monomer, which emits at 390 nm, and the simultaneous decrease in concentration of the non-hydrolyzed LCAT Substrate, which emits at 470 nm.
          Données d'exemple

          Figure 2: Example Data

          Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample.

          Marques déposées Calbiochem® is a registered trademark of Merck KGaA, Darmstadt.
          Interactive Pathways™ is a trademark of Merck KGaA, Darmstadt.