539484 | Protein Kinase Assay Kit, Non-Radioactive

539484
  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
Demander le prix
Disponibilité limitée Disponibilité limitée
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
      Nous vous tiendrons informé

       

      Contacter le Service Clients

         ComparerClick To Print This Page

      Overview

      Tableau de caractéristiques principal

      Méthodes de détection
      Colorimetric
      Description
      Aperçu Useful for measuring protein kinase A (PKA) or protein kinase C (PKC) activity in crude cell extracts or semi-purified enzyme preparations. The assay kit is based on an ELISA method which utilizes a synthetic PKA/PKC pseudosubstrate and a monoclonal antibody which recognizes the phosphorylated form of that peptide. May be used for screening inhibitors and activators of PKA and/or PKC. Supplied with a user protocol.
      Référence 539484
      Famille de marques Calbiochem®
      Références bibliographiques
      Références bibliographiques Newton, A.C. 1995. J. Biol. Chem. 270, 28495.
      Nishizuka, Y. 1995. FASEB J. 9, 484.
      Nishizuka, Y. 1988. Nature 334, 661.
      Edelman, A.M., et al. 1987. Ann. Rev. Biochem. 56, 567.
      Nishizuka, Y. 1986. Science 233, 305.
      Montimity, M.R. and Bilezikijan, L.M. 1987. Nature 56, 175.
      Inagaki, M., et al. 1985. J. Biol. Chem. 260, 2922.
      Wolf, M., et al. 1985. Nature 317, 546.
      Beavo, J.A., et al 1974. Methods Enzymol. 38, 299.
      Informations produit
      Méthode de détection Colorimetric
      Déclaration Not available for sale in Japan.
      Forme 96 Tests
      Format 96-well plate
      Contenu du kit 96-Well Substrate-Coated Plate, Sample Preparation Buffer, Reaction Buffer, Calcium Solution, Phosphatidylserine, Detector Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solutions, Stop Solution, and a user protocol.
      Applications
      Informations biologiques
      Type d'échantillon Cell lysates, purified enzymes
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Phrase R (de risques) R: 20/21/22-34-36/37/38-42/43-45





      Phrase S (de sécurité) S: 26-36/37/39-45-3-28





      Notifications sur l'utilisation du produit
      Utilisation prévue The Calbiochem® Protein Kinase Assay Kit, Non-Radioactive is intended for the qualitative determination of cytosolic protein kinase C (PKC) and/or protein kinase A (PKA) using cell lysates or purified enzymes. The kit is suitable for screening of inhibitors and activators of PKA and/or PKC , monitoring the purification process for PKA or PKC, and measuring the effect of various pharmacological agents on PKC and/or PKA activity in biological systems. Please note that translocation of PKC from the cytosol to the membrane is a hallmark of PKC activation for most PKC isoforms. Since this kit measures cytosolic PKC rather than membrane-associated PKC, there may be a decrease in cytosolic PKC activity in cell samples that have been activated. The kit is not suitable for determination of calcium or phospholipid dependency with purified PKC.

      NOTE: Please review the entire protocol before commencing with the assay. The Protein Kinase Assay Kit, Non-Radioactive has been tested and optimized for measuring either PKC or PKA activity using cell lysates from FM3A cells.
      Informations relatives au stockage et à l'expédition
      Code Expé Blue Ice Only
      Toxicité Multiple Toxicity Values, refer to MSDS
      Attention matériaux dangereux : Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Stockage +2°C to +8°C
      Ne pas congeler No
      Informations sur l'emballage
      Informations sur le transport
      Information complémentaire
      Contenu du kit 96-Well Substrate-Coated Plate, Sample Preparation Buffer, Reaction Buffer, Calcium Solution, Phosphatidylserine, Detector Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solutions, Stop Solution, and a user protocol.
      Caractéristiques

      Documentation

      FDS

      Langues
      Danmark Deutschland Ellas
      English España France
      Italia Korea Magyarország
      Nederland  Norge Polska
      Portugal Suomi Sverige
      България

      Certificats d'analyse

      TitreNuméro de lot
      539484

      Références bibliographiques

      Aperçu de la référence bibliographique
      Newton, A.C. 1995. J. Biol. Chem. 270, 28495.
      Nishizuka, Y. 1995. FASEB J. 9, 484.
      Nishizuka, Y. 1988. Nature 334, 661.
      Edelman, A.M., et al. 1987. Ann. Rev. Biochem. 56, 567.
      Nishizuka, Y. 1986. Science 233, 305.
      Montimity, M.R. and Bilezikijan, L.M. 1987. Nature 56, 175.
      Inagaki, M., et al. 1985. J. Biol. Chem. 260, 2922.
      Wolf, M., et al. 1985. Nature 317, 546.
      Beavo, J.A., et al 1974. Methods Enzymol. 38, 299.

      Brochure

      Titre
      Protein Kinase Assay and Detection Kits Brochure
      Protein Kinase and Related Tools Brochure

      Protocoles Utilisateur

      Titre
      User Protocol