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MABN640 Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody

MABN640
50 µg  
Purchase on Sigma-Aldrich

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Panoramica

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Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H DB, WB, IHC Rb Purified Monoclonal Antibody
Description
Catalogue NumberMABN640
DescriptionAnti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody
Alternate Names
  • Amyloid Beta
  • Amyloid B
  • Amyloid beta A4 protein
  • ABPP
  • APPI
  • APP
  • Alzheimer disease amyloid protein
  • Cerebral vascular amyloid peptide
  • CVAP
  • PreA4
  • Protease nexin-II
  • PN-II
  • N-APP2
  • Soluble APP-alpha
  • S-APP-alpha
  • Soluble APP-beta
  • S-APP-beta
  • C995.Beta-amyloid protein 42
  • Beta-APP42
  • Beta-amyloid protein 40
  • Beta-APP40
  • C83
  • P3(42)
  • P3(40)
  • C80
  • Gamma-secretase C-terminal fragment 59
  • Amyloid intracellular domain 59
  • AICD-59
  • AID(59)
  • Gamma-CTF(59)
  • Gamma-secretase C-terminal fragment 57
  • Amyloid intracellular domain 57
  • AICD-57
  • AID(57)
  • Gamma-CTF(57)
  • Gamma-secretase C-terminal fragment 50
  • Amyloid intracellular domain 50
  • AICD-50
  • AID(50)
  • Gamma-CTF(50)
  • C31
Background InformationThe protein named Amyloid beta A4, or ABPP, APP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, or Protease nexin II (PN-II) protein and encoded by human gene A4/AD1 is a precursor to 14 peptides some of which are associated with neurological disease such as Alzheimer's Disease. In pathological conditions Amyloid beta A4 protein is the precursor for the disease associated proteins, Beta amyloid 40, and Beta amyloid 42, major components of plaques in Alzheimer’s Disease. Normally APP plays a role in neuronal development and mediates aspects of axonal transport as well as neurite outgrowth. It is also involved in copper homeostasis/oxidative stress through copper ion reduction in neurons. Amyloid Beta A4 protein is localized to the plasma membrane and Golgi complex until cleaved. Amyloid Beta A4 protein is normally expressed in all fetal tissues and in the adult, frontal cortex expression predominates although there are isoforms widely expressed in non-neuronal cells as well. Normally, amyloid beta A4 is proteolytically processed and the cleavage and secretion of fragments are non-amyloidogenic. However under presenilin/nicastrin mediated processing of the C99 fragment releases beta amyloid proteins that are associated with neurological disease. MABN640 recognizes a specific conformation of residues 2-7 of the amyloid Aß peptide sequence (AEFRHD) found in amyloid fibrils. It does not detectably react with APP, IAPP, a-synuclein or polyQ fibrils A different antibody MABN637, recognizes a specific conformation of residues 3-6 of the amyloid Aß peptide sequence (EFRH) found in amyloid fibrils. It does not recognize pyroglutamyl Aß at residue 3 (1). MABN640 displays a distinct specificity from MABN638 on dot blots of Aß preparations (1). It also reacts with polyQ40 fibrils but not monomers, indicating that it recognizes a generic, sequence independent epitope.
References
Product Information
FormatPurified
PresentationPurified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationThis Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody is validated for use in Dot Blot and Western Blotting and Immunohistochemistry for the detection of Amyloid beta fibrils.
Key Applications
  • Dot Blot
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 0.1 µg/mL from a representative lot detected Amyloid beta fibrils in Abeta42 aggregates (Data courtesy of Prof. C. Glabe, UCI).
Immunohistochemistry Analysis: A representative lot detected Amyloid beta fibrils in Frontal cortex of AD patients (Data courtesy of Prof. C. Glabe, UCI).
Biological Information
ImmunogenCorresponding to the Beta Amyloid Protein 42 (residue 2-7) of human Amyloid beta fibrils.
EpitopeBeta Amyloid Protein 42 (residue 2-7)
CloneM98
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityMABN640 recognizes a specific conformation of residues 2-7 of the amyloid Aß peptide sequence (AEFRHD) found in amyloid fibrils. It does not detectably react with APP, IAPP, a-synuclein or polyQ fibrils (Hatami et al., manuscript in preparation). This antibody reconizes both Abeta40 and ABeta42.
IsotypeIgGκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rabbit, Bovine, Chicken based on 100% sequence homology. Other homologies: Broad species cross-reactivity is expected.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APP
  • A4
  • AD1
Purification MethodProtein A Purfied
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot in Synthetic Abeta40.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected Amyloid beta fibrils in 0.075 µg of Synthetic Abeta40.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal - Q2437459Q2437459

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Positron emission tomography imaging of fibrillar parenchymal and vascular amyloid-β in TgCRND8 mice.
McLean, Daniel, et al.
ACS Chem Neurosci, 4: 613-23 (2013)  2013

Mostra il sommario
23509918 23509918
Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β.
Nussbaum, Justin M, et al.
Nature, 485: 651-5 (2012)  2012

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