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3465 | LIGHT DIAGNOSTICS™ Enterovirus Screening Set Kit (Coxsackie A9 mAb, Echovirus Blend, Enterovirus Blend, Coxsackie Blend, Poliovirus Blend, Pan-Enterovirus Reagent, IgG FITC secondary, Pan-Enterovirus Control Slides, Normal Mouse Antibody, Mounting & Wash Solutions)

3465
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      Key ApplicationsFormatHostDetection Methods
      IF Purified M Fluorescent
      Description
      Catalogue Number3465
      Trade Name
      • LIGHT DIAGNOSTICS
      • Chemicon
      DescriptionLIGHT DIAGNOSTICS™ Enterovirus Screening Set Kit (Coxsackie A9 mAb, Echovirus Blend, Enterovirus Blend, Coxsackie Blend, Poliovirus Blend, Pan-Enterovirus Reagent, IgG FITC secondary, Pan-Enterovirus Control Slides, Normal Mouse Antibody, Mounting & Wash Solutions)
      OverviewENTEROVIRUS SCREENING SET KIT

      Cat. No., Antigen, Subclasses, Quantity, Specificity

      3301, Coxsackie A9 monoclonal antibody, 1.0 mL

      3303, Coxsackie B Blend, IgG2a& IgG2b, 2.0 mL, Coxsackie B1, B2, B3, B4, B5 and B6

      3311*, Echovirus Blend, IgG2a& IgG2b, 2.0 mL, Echovirus 4, 6, 9, 11, 30 and 34 (Coxsackie A24)

      3321, Enterovirus Blend, IgG1 & IgG, 2.0 mL, Enterovirus 70 and 71/Coxsackie A16

      3336, Poliovirus Blend, IgG1& IgG2a, 2.0 mL, Poliovirus 1, 2 and 3

      3360**, Pan-Enterovirus Blend, IgG3& IgM, 5.0 mL, Enteroviruses

      5069, Pan-Enterovirus Control Slides - (Catalog No. 5069). Two control slides containing five infected (positive) wells and one non-infected (negative) wells. Each infected well represents a different enterovirus: Coxsackievirus A9, coxsackievirus B2, echovirus 30, enterovirus 71 and poliovirus 2. There is one uninfected (negative) well.

      5014, Normal Mouse Antibody – (Catalog No. 5014). One 10 ml dropper vial containing normal mouse antibody to be used as a negative control.

      5008, Anti-Mouse IgG:FITC – (Catalog No. 5008). One 10 ml dropper vials containing FITC labeled goat anti mouse IgG.

      5087, Phosphate Buffered Saline - (Catalog No. 5087). One packet of Phosphate Buffered Saline Salts yields 1 liter when dissolved in distilled water. Store in a clean closed container at room temperature.

      5037, Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037). One 10 ml vial containing polyoxyethylene sorbitan monolaurate (Tween 20) and sodium azide (NaN3) concentrate to be diluted 1:100 in PBS.

      5013, Mounting Fluid - (Catalog No. 5013). One 10 mL dropper vial containing Tris-buffered glycerin, a fluorescence enhancer, and 0.1% sodium azide (preservative).

      * Echovirus 4 and thereby the Echovirus Blend may give some weak cross reactivity with Poliovirus 1, 2 and 3. It is recommended to test samples weakly reactive with Echovirus Blend with Light Diagnostics Poliovirus Blend, (Catalog No. 3336) to ensure proper identification.

      ** Cross reactivity with other viruses may be possible. If low level nonspecific staining is seen and interferes with interpretation of the Pan-Enterovirus IFA results the nonspecific stain can be eliminated by pre-incubating the sample slide/well with PBS, .05% Tween 20, .02% sodium azide with 1% casein or 1% Difco Bacto Skim Milk for 15-30 minutes at 37*C. Pre-incubation will not effect the specific Enterovirus activity.

      The Coxsackie A9 mAb and the Coxsackie B, Echovirus, Enterovirus, Pan-Enterovirus Blend and Poliovirus Blends are intended for in vitro diagnostic use in indirect fluorescence screening for the presumptive identification of viral isolates obtained in culture.
      Materials Required but Not Delivered1. Cell culture for isolation of respiratory viruses: Each laboratory must maintain viable stocks of cells at appropriate passage state that will efficiently allow replication of respiratory viruses from processed patient specimens. These cells must be checked periodically for ability to support growth of respiratory viruses. Appropriate cell lines can be obtained from the American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville, MD 20852.
      2. Viral transport medium (VTM), which is non-inhibitory to the respiratory viruses and the tissue culture cells used for viral isolation: Hank's balanced salt solution (HBSS) with antibiotics and a protein stabilizer is a suitable medium. Avoid use of animal sera (except precolostral fetal bovine serum (FBS)) as protein stabilizer to prevent interference from inherent antibody.
      3. Tissue culture media such as RPMI or Eagle's Minimum Essential Medium (EMEM) with appropriate amount of precolostral FBS can be used.
      4. Sterile tissue culture tubes, dram vials, or multi well plates
      5. Acetone, reagent grade or better
      Note: Acetone is hygroscopic and should be kept in tightly stoppered bottles. Presence of moisture in the acetone may result in a hazy appearance on the substrate during fluorescence microscopy.
      6. Acetone-cleaned glass slides with wells at least 6mm in diameter in a hydrophobic mask
      7. Sterile pipettes
      8. Humid chamber
      9. Sodium hypochlorite solution (0.05%)
      10. No. 1 coverslips
      11. 37°C incubator with rheostat for temperature regulation
      12. Sterile swabs
      13. Forceps
      14. Vials for collection and transportation of specimens
      15. Fluorescence microscope with appropriate filter combination for fluorescein (excitation peak 490 nm, emission peak 520 nm)
      16. Sterile glass beads (1 3 mm diameter)
      17. Centrifuge
      18. Vortex mixer or sonicator
      19. Distilled or deionized water
      References
      Product Information
      Components
      • Coxsackie A9 monoclonal antibody-(Catalogue No. 3301)
      • Coxsackie B Blend-(Catalogue No. 3303)
      • Echovirus Blend-(Catalogue No. 3311)
      • Enterovirus Blend-(Catalogue No. 3321)
      • Poliovirus Blend-(Catalogue No. 3336)
      • Pan-Enterovirus Reagent- (Catalogue No. 3360)
      • Pan-Enterovirus Control Slides (2)- (Catalog No. 5069). Two control slides containing five infected (positive) wells and one non-infected (negative) wells. Each infected well represents a different enterovirus: Coxsackievirus A9, coxsackievirus B2, echovirus 30, enterovirus 71 and poliovirus 2. There is one uninfected (negative) well.
      • Normal Mouse Antibody – (Catalog No. 5014).
      • Anti-Mouse IgG:FITC – (Catalog No. 5008).
      • Phosphate Buffered Saline - (Catalog No. 5087).
      • Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037).
      • Mounting Fluid - (Catalog No. 5013).
      Detection methodFluorescent
      FormatPurified
      PresentationPre-diluted "Ready to Use".
      Applications
      Key Applications
      • Immunofluorescence
      Application NotesATTENTION:

      It is strongly recommended that first time Enterovirus Screening Set Kit, (Cat No. 3465) users request package inserts which contain the specific performance characteristics of each reagent.
      Biological Information
      Immunogenvirus antigen
      HostMouse
      Specificityeach reagent is specific to an enterovirus type
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Radioactive Isotypeunlabeled, IFA format
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For in vitro Diagnostic Use
      • CE Mark
      Storage and Shipping Information
      Storage ConditionsWhen stored at 2-8°C, the SimulFluor® Respiratory Screen is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagent after the kit expiration date.

      Warnings and Precautions
      • Sodium azide (present in the conjugate, monoclonal antibodies, wash buffer, and mounting fluid) can react with lead or copper plumbing to form potentially explosive metal azides. When disposing of these materials, flush with large volumes of water to prevent azide build-up.
      • Pooling or diluting conjugates or monoclonal antibodies may cause erroneous results.
      • Do not allow slides to dry at any time during the staining procedure.
      • Handle all specimens and materials as if potentially infectious. Decontaminate with 0.05% sodium hypochlorite (a 1:100 dilution of household bleach) prior to disposal.
      • Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks, or flames. Avoid breathing vapor. Use adequate ventilation.
      • Do not mouth pipette reagents.
      • Do not substitute reagents from other manufacturers.
      • Alteration of protocol provided may cause erroneous results.
      • When staining multiple samples on a slide, avoid cross contamination between samples.
      • Mounting Fluid contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications