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CBL480-KC | Mouse Anti-Human CD42B, clone MM2/174 (1mg) KC

CBL480-KC
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      Description
      Catalogue NumberCBL480-KC
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMouse Anti-Human CD42B, clone MM2/174 (1mg) KC
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti- CD42B
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationUsed in studies involving von Willebrand's factor binding to GPIb
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesFunctional studies involving von Willebrand's factor binding to GPIb

      Flow cytometry

      Immunofluorescence studies of platelets, reacts with GPIb molecule

      Immunohistochemistry studies with platelets and megakaryocytes in frozen sections

      Formalin fixed decalcified sections of bone marrow. Megakaryocytes and platelets are labeled without pretreatment and with pretreatment using protease type XXIV (0.5mg/ml). HIER (Microwave, citrate buffer pH 6.0) may give superior results.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman plasma membrane
      CloneMM2/174
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody is directed against the CD42b antigen, platelet glycoprotein GPIb that serves as a receptor for von Willebrand factor and as a high affinity thrombin receptor. CBL 480 immunoblots a band of 130 kDa on SDS gels of platelet lysates and gives surface immunofluorescence with fixed or unfixed platelets. The antigen is absent or present in very low levels on platelets of patients with the Bernard-Soulier syndrome.

      This antibody also recognises megakaryocytes in bone marrow

      FUSION PARTNER: SP2/0 myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that are linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.
      Gene Symbol
      • GP1BA
      • MGC34595
      • CD42B
      • GP1B
      • GPIb-alpha
      • BSS
      • CD42b-alpha
      • GPIbA
      • CD42b
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07359 # GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of von Willebrand factor, which is already bound to the subendothelium.
      SIZE: 626 amino acids; 68955 Da
      SUBUNIT: Heterodimer composed of GP-Ib alpha and beta; disulfide linked. GP-IX is complexed with the GP-Ib heterodimer via a non covalent linkage. Interacts with FLNB.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis.
      DISEASE: SwissProt: P07359 # Defects in GP1BA are a cause of Bernard-Soulier syndrome (BSS) [MIM:231200]; also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency. & Defects in GP1BA are the cause of benign mediterranean macrothrombocytopenia [MIM:153670]; also known as autosomal dominant benign Bernard-Soulier syndrome. Benign mediterranean macrothrombocytopenia is characterized by mild or no clinical symptoms, normal platelet function, and normal megakaryocyte count. & Defects in GP1BA are a cause of von Willebrand disease (vWD) [MIM:177820]; also known as platelet-type von Willebrand disease or pseudo-von Willebrand disease (pseudo-vWD). This autosomal dominant bleeding disorder is caused by an increased affinity of GP-Ib for soluble vWF resulting in impaired hemostatic function due to the removal of vWF from the circulation. & Genetic variations in GP1BA may be a cause of susceptibility to nonarteritic anterior ischemic optic neuropathy (NAION) [MIM:258660]; also known as susceptibility to anterior ishcemic optic neuropathy (AION). AION involves loss of vision due to damage to the optic nerve from insufficient blood supply. AION is generally divided into two types: arteritic AION and NAION. NAION probably results from minute infarctions of the optic nerve caused by occlusion of the posterior ciliary arteries. Hypercholesterolemia, diabetes mellitus, ischemic heart disease, hyperhomocysteinemia, hypertension, and crowded disk have been implicated as predisposing conditions.
      SIMILARITY: SwissProt: P07359 ## Contains 6 LRR (leucine-rich) repeats.
      MISCELLANEOUS: Platelet activation apparently involves disruption of the macromolecular complex of GP-Ib with the platelet glycoprotein IX (GP-IX) and dissociation of GP-Ib from the actin- binding protein. & Binding sites for von Willebrand factor and thrombin (the latter site with unknown function) are in the amino- terminal part of the molecule.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Prodotti e applicazioni correlate

      Prodotti correlati per: Application Facete

      Prodotti correlati per: Brand Facete

      Categorie

      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Other Primary Bulk Antibodies
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Secondary Bulk Antibodies > Anti-Human Antibodies & Conjugates > Mouse Anti-Human
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Tumor Markers & Antigens
      Life Science Research > Antibodies and Assays > Primary Antibodies