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GF091-K | SRANK-LIGAND, HU

GF091-K
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      Replacement Information
      Description
      Catalogue NumberGF091-K
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionSRANK-LIGAND, HU
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Background InformationRANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.
      References
      Product Information
      FormatPurified
      PresentationSterile filtered, and then lyophilized from 5 mM sodium phosphate, pH 7.6 + 75 mM NaCl. Reconstitute with distilled water to a concentration of 0.1-1 mg/mL as a stock solution. Solution can then be diluted into other aqueous buffers for immediate use.
      Applications
      ApplicationThe recombinant human sRANKL protein is produced in E. Coli.
      Key Applications
      • Cell Culture
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
      SourceE. coli
      Specific ActivityBIOLOGICAL ACTIVITY: <br />Determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0-10.0 ng/mL.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
      Gene Symbol
      • TNFSF11
      • RANKL
      • hRANKL2
      • ODF
      • OPGL
      • CD254
      • OPGLG
      • sOdf
      • OPTB2
      • TRANCE
      UniProt SummaryFUNCTION: SwissProt: O14788 # Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
      SIZE: 317 amino acids; 35478 Da
      SUBUNIT: Homotrimer (By similarity).
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type II membrane protein. & Isoform 3: Cell membrane; Single-pass type II membrane protein. & Isoform 2: Secreted (By similarity). & Processed isoform 1: Secreted (By similarity).
      TISSUE SPECIFICITY: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
      PTM: The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
      DISEASE: "SwissProt: O14788 # Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non- functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development."
      SIMILARITY: SwissProt: O14788 ## Belongs to the tumor necrosis factor family.
      Stem Cell Type
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsDry Ice (-70°C)
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information
      Specifications

      Prodotti e applicazioni correlate

      Alternative Packsize

      Numero di catalogo Descrizione  
      GF091 Soluble RANK Ligand (sRANKL) Protein, Recombinant human Prezzi e disponibilità

      Prodotti correlati per: Brand Facete

      Categorie

      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Growth Factors
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Arthritis related
      Life Science Research > Proteins and Enzymes > Other Proteins