70561 pET-42a(+) DNA - Novagen

70561
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      Replacement Information

      Prezzi e disponibilità

      Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
      70561-3
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          Fiala di plastica 10 μg
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          Description
          OverviewThe pET-41a-c(+) and pET-42a-c(+) vectors incorporate the schistosomal glutathione-S-transferase (GST; GST•Tag) coding sequence as a fusion partner. The GST•Tag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners (Smith 1998). When expressed in a soluble, properly folded form, GST•Tag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. The pET-41 and -42 series feature the powerful T7lac promoter, and encode the GST•Tag (220 aa) sequence, proteolytic sites, His•Tag (6 aa) sequence, and S•Tag (15 aa) sequence.

          In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow removal of 100% of the vector-encoded sequences and the generation of native proteins with their authentic N-terminal residues. Unfused proteins can be produced by using the Nde I cloning site. A version of pET-41 is available as a linearized vector prepared for ligation-independent cloning (LIC) of PCR products.

          Another pET vector with the GST•Tag sequence is pET-49b(+). Please see the website description for more information. The His•Tag and S•Tag sequences enable alternative protein detection and purification procedures to be performed. For example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.



          The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB239). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number70561
          Brand Family Novagen®
          References
          References

          Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31.

          Product Information
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
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          Supplemental Information
          Specifications

          Documentation

          pET-42a(+) DNA - Novagen MSDS

          Titolo

          Scheda di sicurezza (MSDS) 

          pET-42a(+) DNA - Novagen Certificati d'Analisi

          TitoloNumero di lotto
          70561

          Riferimenti bibliografici

          Panoramica delle referenze

          Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31.

          Brochure

          Titolo
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

          Citazioni

          Titolo
        • F. Allemand, et al. (2007) Escherichia coli ribosomal protein L20 binds as a single monomer to its own mRNA bearing two potential binding sites. Nucleic Acids Research 35, 3016-3031.
        • Rutilio A. Fratti, et al. (2007) Stringent 3Q·1R composition of the SNARE 0-layer can be bypassed for fusion by compensatory SNARE mutation or by lipid bilayer modification. Journal of Biological Chemistry 282, 14861-14867.
        • Jeffrey G. Gardner, et al. (2006) Control of acetyl-coenzyme A synthetase (acsA) activity by acetylation/deacetylation without NAD+ involvement in Bacillus subtilis. Journal of Bacteriology 188, 5460-5468.
        • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
        • Tao Wu, et al. (2006) Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli. Procedings of the National Academy of Science 103, 11754-11759.
        • Karthikeyan Kandasamy, et al. (2005) Translational control of 2-adrenergic receptor mRNA by T-cell-restricted intracellular antigen-related protein. Journal of Biological Chemistry 280, 1931-1943.
        • Irene Soderhall, et al. (2005) An ancient role for a prokineticin domain in invertebrate hematopoiesis. journal of Immunology 174, 6153-6160.
        • Junji Yamauchi, et al. (2005) The neurotrophin-3 receptor TrkC directly phosphorylates and activates the nucleotide exchange factor Dbs to enhance Schwann cell migration. Proceedings of the National Academy of Sciences (USA) 102, 5198-5203.
        • Brian A. Zabel, Amanda M. Silverio and Eugene C. Butcher. (2005) Chemokine-like receptor 1 expression and chemerin-directed chemotaxis distinguish plasmacytoid from myeloid dendritic cells in human blood. Journal of Immunology 174, 244-251.
        • Protocolli per l'uso

          Titolo
          TB055 pET System Manual

          Mappa di un vettore

          Titolo
          TB240VM pET-42a-c(+) Vector Map

          Prodotti e applicazioni correlate

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          Numero di catalogo Descrizione  
          70556 pET-41a(+) DNA - Novagen Prezzi e disponibilità
          70557 pET-41b(+) DNA - Novagen Prezzi e disponibilità
          70562 pET-42b(+) DNA - Novagen Prezzi e disponibilità

          Categorie

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors