Applications – Internalization

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Featured Spotlights
Detection of internalized exosomes by tumor cells using Amnis® ImageStreamX
  Technical Information:
Quantitation of gamma-H2AX spots on the ImageStream (09-008)


Merck:/Freestyle/BI-Bioscience/Cell-Analysis/amnis/Amins2-images/internalization2.jpgCellular processes such as drug uptake and processing by cells, entry of pathogens and toxins, or antigen processing and presentation are studied on Amnis® imaging flow cytometers by measuring internalization. Amnis® imaging flow cytometry systems automatically collect large numbers of images per data set and provides quantitative image analysis tools that facilitate the objective and rapid localization of internalized probes. Because the ImageStream®collects multiple fluorescent images per cell, internalized markers in phenotypically distinct sub-populations as well as co-localization to different cellular compartments (see Co-localization) can be simultaneously determined in a quantitative manner.

Featured Video

Watch to learn how multispectral imaging in flow can be used to answer specific internalization research questions. Dr. Sherree Friend explains how Amnis® applications use high-throughput imaging to observe primary dendritic cells to provide insight regarding cellular internalization of antigen and other particles.

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Phagocytosis by Murine Macrophages

Internalization of zymosan (green) by murine RAW cells (orange) identified by immunophenotyping. Phagocytosis is measured as the percentage of cells with internalized zymosan at 15, 30 and 60 minutes at 37 degrees C.

Internalization of CpGB in Primary Plasmacytoid Dendritic Cells Detail

Binding, internalization, and co-localization to of CpGB (red) to lysosomes (green) by plasmacytoid dendritic cells (pDC, orange) is measured using the ImageStreamX. This data highlights the following capabilities of the ImageStream: 1) the combination of immunophenotyping (pDC identification via fluorescent intensity staining) with image correlation and internalization analysis, allowing measurement of co-localization and internalization of molecules within specific subsets, and 2) the ability to measure these events in small, primary cells with small cytoplasmic compartments.

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