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566415 PhosphoDetect™ Anti-Smad2 (pSer465/467) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phospho-MADR2

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, Mi, MRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~58 kDa Smad2 protein phosphorylated at Ser465/467.
      Catalogue Number566415
      Brand Family Calbiochem®
      SynonymsAnti-Phospho-MADR2
      Application Data
      Detection of human Smad2, phosphorylated on Ser465/467, by immunoblotting. Sample: Whole cell lysates from HepG2 cells treated with TGF-β for 0-60 min. Primary antibodies: PhosphoDetect™ Anti-Smad2 (pSer465/467) Rabbit pAb (Cat. No. 566415) (1:1000) (upper panel) or total Anti-Smad2 (lower panel). Detection: chemiluminescence.
      References
      ReferencesHeldin, C.H., et al. 1997. Nature 390, 465.
      Wrana, J. and Pawson, T. 1997. Nature 388, 28.
      Marcias-Silva, M., et al. 1996. Cell 87, 1215
      Massague, J., et al. 1996. Cell 85, 947.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES sodium, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000, see comments)
      Application CommentsThe immunogen seqeunce is conserved in chicken, human, mouse, rat, Xenopus, Zebrafish, and Drosophila. Does not cross-react with other Smad related proteins. For immunoblotting, incubate with antibody diluted in 1x TBS, 0.1% Tween®-20 detergent, and 5% dry milk overnight at 4°C with gentle shaking. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide surrounding the Ser⁴⁶⁵ and Ser⁴⁶⁷ phosphorylation sites of human Smad2
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mink
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      566415 0

      Documentation

      PhosphoDetect™ Anti-Smad2 (pSer465/467) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Heldin, C.H., et al. 1997. Nature 390, 465.
      Wrana, J. and Pawson, T. 1997. Nature 388, 28.
      Marcias-Silva, M., et al. 1996. Cell 87, 1215
      Massague, J., et al. 1996. Cell 85, 947.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      SynonymsAnti-Phospho-MADR2
      ApplicationImmunoblotting (1:1000, see comments)
      Application Data
      Detection of human Smad2, phosphorylated on Ser465/467, by immunoblotting. Sample: Whole cell lysates from HepG2 cells treated with TGF-β for 0-60 min. Primary antibodies: PhosphoDetect™ Anti-Smad2 (pSer465/467) Rabbit pAb (Cat. No. 566415) (1:1000) (upper panel) or total Anti-Smad2 (lower panel). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody purified by protein A followed by immunoaffinity chromatography. Recognizes the ~58 kDa Smad2 protein phosphorylated at Ser465/467.
      BackgroundThe MAD-related (MADR) family of proteins are intracellular signaling components for the TGFβ superfamily of ligands. Smad2 is activated by phosphorylation by the TGFβ/activin receptor. Activated Smad2 forms a heterocomplex with Smad4 that is then translocated into the nucleus, where it associates with other transcription factors and regulates expression of ligand-responsive genes. Both Smad2 and its closely related homolog Smad3 act as latent nuclear transcriptional activators and mediate intracellular signaling by TGF-betas and activin.
      HostRabbit
      Immunogena synthetic phosphopeptide surrounding the Ser⁴⁶⁵ and Ser⁴⁶⁷ phosphorylation sites of human Smad2
      IsotypeIgG
      Specieshuman, mink, mouse
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES sodium, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsThe immunogen seqeunce is conserved in chicken, human, mouse, rat, Xenopus, Zebrafish, and Drosophila. Does not cross-react with other Smad related proteins. For immunoblotting, incubate with antibody diluted in 1x TBS, 0.1% Tween®-20 detergent, and 5% dry milk overnight at 4°C with gentle shaking. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHeldin, C.H., et al. 1997. Nature 390, 465.
      Wrana, J. and Pawson, T. 1997. Nature 388, 28.
      Marcias-Silva, M., et al. 1996. Cell 87, 1215
      Massague, J., et al. 1996. Cell 85, 947.

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