SCC150 | 16HBE14o- Human Bronchial Epithelial Cell Line

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      概要

      Replacement Information
      Description
      Catalogue NumberSCC150
      Description16HBE14o- Human Bronchial Epithelial Cell Line
      Alternate Names
      • 16HBE
      • 16-HBE
      • 16HBEo-
      • 16-HBEo
      • 16-HBE14o
      Background Information16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport (1). When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein (1). Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.

      References:
      1. Cozens AL, et al. (1994) CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells. Am J Respir Cell Mol Biol. 10(1): 38-47.
      2. Kunzelmann K, et al. (1994) cAMP-dependent activation of ion conductances in bronchial epithelial cells. Pflugers Arch 428(5-6): 590-596.
      3. Koslowsky T, et al. (1994) Ca(2+)- and swelling-induced activation of ion conductances in bronchial epithelial cells. Pflugers Arch 428(5-6): 597-603.
      4. Wan H, et al. (2000) Tight junction properties of the immortalized human bronchial epithelial cells Calu-3 and 16HBE14o-. Eur Respir J. 15(6): 1058-1068.
      5. Illek B, et al. (2010) Sensitivity of chloride efflux vs. transepithelial measurements in mixed CF and normal airway epithelial cell populations. Cell Physiol Biochem 26(6): 983-990.
      References
      Product Information
      Applications
      Application16HBE14o- human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications

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